Haploidentical Hematopoietic Stem Cell Transplantation Combined With Donor-Derived Cytokine-Induced Killer Cells Successfully Treated The Patients With Hematologic Malignancies Relapsing After Autologous Stem Cell Transplantation

Objective: To investigate the effect of haploidentical hematopoietic stem cell transplantation combined with donor-derived cytokine-induced killer(CIK) cells treating the patients with hematologic malignancies relapsing after autologous stem cell transplantation. Methods: One acute myeloid leukemia patient and one T lymphoblastic lymphoma/acute T lymphoblastic leukemia patient relapsed post the conventional chemotherapies and autologous stem cell transplantation. We applied the haploidentical hematopoietic stem cell transplantation immediately. Consequently, using cell separator to obtain the mobilized peripheral blood mononuclear cells of donors, then CIK cells were expanded under the stimulation with IFN-γ, IL-2, OKT3. Results: The process of two patients engrafted successfully, they had hemorrhagic cystitis during the process of transplantation. After repeatly infusions of cytokine-induced killer cells, graft-versus-host disease of patients didn't aggravate. Their bone marrows keep continuous complete remission. Conclusions: The major cytotoxic effectors cells of cytokine-induced killer cells are CD3 positive, CD56 positive, and CD8 positive cells with interferon-γ. They could eliminate the residual disease and prolong disease free survival time, without exacerbating graft-versus-host disease. Further experiences in additional patients with longer follow-up are necessary.