Fundamental Studies On Chemical Constituents Of Brassica RapaL.

Objective:The present paperconducts conducts the research exactly to the Akeshu habitats of Brassica rapaL medicine hemp ancient 75% ethyl alcohol extractions chemical composition.Intends to exposition exactly hemp ancient the effective component,accumulation enough biomass,to hemp ancient the biological activity research provide the matetial base exactly.And the carbohydrate and the flavanone class material's content carries on the determination to exactly hemp ancient in.determine the content of sugar and flavone.Methods:1.This article through uses the solvent extraction and many kinds of chromatography(Including sillica. gel column chromatography law,pocket polymeric adsorbent chromatography,glucosan gelatin methods and so on column chromatography law and heavy crystallization)carry on the chemical composition to the Aqsu habitat exactly hemp ancient 75% ethyl alcohol extraction's benzine part and the normal butyl alcohol part the separation to purify and to obtain the monomer compound.Through('H-NMR,13C-NMR)the compound which obtains to the separation carries on the structure analysis appraisal using the modern spectrum technology.2.Uses in phenlo-sulfuric acid method determination exactly hemp ancient the total sugar content.3.Uses in HPLC law determination exactly hemp ancient the flavanone content.Results:1.From exactly hemp ancient 75% ethyl alcohol extraction benzine part and normal tutyl alcohol fractionation 6 monomer compound According to the physics and chemistry nature examination and the spectral data analysis,and unifies the known compound magnetic resonance data contrastive analysis which in the literature reported,has appraised 6 compound structures,respectively isβ-sitosterol(1),Cetane(2),β-sitosterol-3-O-glucoside(3),quercetin(4),quercetin-β-D-glucopyranoside(5) n-dotriacontylalcohol(6)and the comound (2) (3) (5) (6) obtain for the first time from this plant.Conclusion:1.4 kind of compound to the separation obtain exactly for the first time from Uygur medicine hemp ancient.2.Had proven through the experimental result determines the Uygur medicine hemp ancient total sugar with the phenol-sulfuric acid the content is exactly finally stable reliable,the easy and feasible determination method.3.Had proven through the experimental result the HPLC law determination ancient flavanone's content is one kind of simplicity of operator exactly, stable feasible method.