| The Colorectal Carcinoma is a kind of common gastrointestinal cancer, the incident rate is up-trending obviously. The global incidence of Colorectal Carcinoma is occupied in third position of malignant tumor, almost 1 million individuals will develop it every year, and at least 50 thousand patients dead. In our country, the Colorectal Carcinoma is also one of the most familiar malignant tumor, the incident rate is second only to lung cancer and breast cancer in the malignant tumor, the mortality rate is located in the fouth. Currently, as the early diagnosis's level for Colorectal Carcinoma is remain low, around more than 60% suffers have already been in the advancing upon the explicit diagnosis, and only 2/3 suffers can accept to do radical surgery, half of them appreared to relapse and metastasis after surgery,5-year survival rate is only 50%. Therefore, the early diagnosis, the comprehensive evaluation of condition and standardized treatment for the Colorectal Carcinoma is still a hard task for nowadays and later. For the first time, in 1997, Ambrosini etc. cloned Survivin gene from human genome library, which encoded protein has the most simply structure and is very special in IAP family, is the strongest apoptosis inhibiting factor in the current finding, called survivin or survive protein, which has function of inhibiting cell apoptosis, adjusting cellular proliferation and inducing angiogenesis. The specific of Survivin is over-expressed in most human tissue of tumor, however, there's no positive expression in the corresponding normal tissue and mesengium, hopefully to become a new target point in diagnosis and treatment of tumor. Bcl-2, as the first discovered apoptosis inhibiting factor, is the main apoptosis inhibiting molecule of Bcl-2 family's proteins, existed in some normal tissues, like immune, nervous and endocrine system, however, it's represent low-expression or non-expression in the cells of which are in the progress of apoptosis. It's already verified of bcl-2's over-expression in tumor tissues, which is related to the oncogenesis. Although Survivin and bcl-2 run respective anti-apoptosis function separately, according to the research, this two anti-apoptosis function are cooperative. Perhaps there's some congenerous transcription activate mechanism between them so as to be in conjunction with each other in anti-apoptosis of malignant tumor.Aim to research the expression of Survivin and bcl-2, as well as their relationship, seek for the function of Survivin and bcl-2 about the Colorectal Carcinoma's nosogenesis and biological behavior, provide a new indicator for it's integrated condition evolution and treatment. This experiment selected 40 the Colorectal Carcinoma's tissue samples, operated during 2006 to 2010, from Colorectal Anus Surgical department of the first hospital of Jilin University, as well as some related suffers'normal tissue samples of focus nidus, by the method of immunohistochemisty S-P to examine the expression of Survivin and bcl-2. For blank control by 0.01mol/l PBS of which replaces the first immune body (Survivin and bcl-2) and positive control by known breast cancer's Survivin and Colorectal Carcinoma's bcl-2 positive slice, those which appreared brown grain in Colorectal Carcinoma's cytochylema and cell nucleus is immunohistochemisty positive stain. The experiment data used SPSS 14.0 for the statistical analysis, and adopt X2 examine for qualitative material. The result shows:Survivin expressed obviously difference between in the beside normal tissue which was non-expression and inside the tumor's tissue which expressed 26 cases(90%), the expression has no relationship with the suffers' age, sex and tissue type, but has closely correlation with tumor Duck stage, lymph node metastasis and distant metastasis, the lower of Duck stage, the higher of the suffers' expression rate who have lymph node metastasis and distant metastasis. Compared with in the beside normal tissue and inside the tumor's tissue, the distinction of bcl-2's expression rate is also huge, are respectively 7.5%(3/40) and 90%(36/40), the expression is irrespective with suffers' age, sex, Duck stage, tissue type, lymph node metastasis and distant metastasis. In the 40 Colorectal Carcinoma samples, Survivin positive expression has 26 case, bcl-2 positive expression has 36 case, both positive expression has 26 case, they expressed postive correlatability in Colorectal Carcinoma. Conclusion:1. Survivin can be believed to be one of indicator of Colorectal Carcinoma's early diagnosis and unfavourable prognosis, meanwhile, be deem to be the ideal targeted treatment symbol of Colorectal Carcinoma.2. bcl-2 play a significant role in pathogenesy of Colorectal Carcinoma, its expression's high or low is irrelevant with clinicopathologic parameter of Colorectal Carcinoma.3. the expression in Colorectal Carcinoma of Survivin and bcl-2 has positive correlatability, the combination of both can be considered as reference factor of diagnosis, and provide theory basis for the targeted gene treatment of Colorectal Carcinoma. |
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