Brg1 Regulates Bone Morphogenetic Protein-2 Induced Osteogenic Differentiation

Objectives:To explore the Brgl regulation mechanism in bone morphogenetic protein 2 (BMP-2)-induced osteogenic differentiation, a primarily cultured mouse osteoblast was provided for investigation of biological function and further studies of Brgl.Methods:Mouse calvarial osteoblasts were primarily cultured through collagenase digestion and induced differentiation using 0,50 or 200μg/L BMP2 to select the optimal dose. Transcription time dynamics analysis of Brgl was enforced by RT-PCR and Western blot after BMP-2 treatments; ALP staining was used to evaluate the capability of osteogenic differentiation after BMP-2 treatments. The role of BMP-2 on Brgl mRNA expression level was detected by quantitative RT-PCR and the protein level was detected by Western Blot. Effect of Brgl on BMP-2-induced differentiation was analyzed by Brgl-siRNA; Ad-Dlx5 was obtained by recombinant adenovirus.RESULTS AND CONCLUSION:BMP-2 could induce the osteogenic differentiation of primarily cultured mouse osteoblast, and the optimal BMP2 induction condition was 200μg/L. The expression of Brgl was up-regulated by BMP-2 in primarily cultured mouse osteoblasts. BMP-2 induced the osteogenic differentiation was inhibited by Brgl-siRNA. The expression of Dlx5 was regulated by Brgl. Brgl could regulate the BMP-2-induced osteogenic differentiation of primarily cultured mouse osteoblast through regulating the expression of Dlx5.