| ObjectiveTo explore the mechanism of the treatment of Menopausal syndrome with Catgut Embedding in points with the study on the effect of the expression of Aromatase P450 mRNA in the female Model of Menopausal Syndrome rats'abdominal adipose tissues.MethodsThe female SD rats with ovaries removed, were adopted as the animal model of menopausal pathology, and were divided randomly into ovariectomized model group(A, n=10), catgut embedding in points group(B, n=11), drug group(C, n=10).At the same time, built sham model group(D, n=9)and normal group(E, n=10). Group B was treated with catgut embedding in points, once every 5 days, four times in all. Acupoints chosen in group A were as follows:Guanyuan(CV4), bilateral Sanyinjiao(SP6)and bilateral Shenshu(BL23). Group C was infused with Nilestriol 5ml/kg(lmg/kg)once every 5 days, four times in all. Group A,D and E were infused with water 5ml/kg once every 5 days, four times in all. After being treated 4 times in succession, all of the experimental animals were put to death on the 5th day after the last treatment, and collected serums and samples, The use of The use of real time polymerase chain reaction (RT-PCR) determination of the rats in each group expressed in different abdominal adipose tissues Aromatase P450 mRNA; The effects were evaluated by measuring the change of body weight; Anatomy from belly fat, pituitary, hypothalamus, uterus, unilateral adrenal gland, and precision that check the above calculation of organ wet weight of organ weight coefficient.Result(1) Body weight:The ovariectomized rats control group exhibited a markely weight gain compared with the sham group (P<0.05), ovariectomized model group had comparative effect in increasing body weight compared with other groups; compared with drug group:the embedding group significantly was reduced, there is very significant differences (P<0.01); compared with ovariectomized model group:the increased weight of the embedding group was lower, there is significant differences (P<0.05). compared with drug group:the increased weight of the embedding group was lower, there is significant differences (P<0.05), however, there was no significant difference compared with sham group.(2) Organ weight coefficient:a. Pituitary weight coefficient:Compared with sham group, pituitary weight coefficient of the embedding group, drug group and ovariectomized model group were reduced, there was very significant differences (P<0.01);among them, ituitary weight coefficient of normal group was the highest, ovariectomized model group was the lowest.b. Weight coefficient of the hypothalamus:Compared with sham group, weight coefficient of the hypothalamus of the embedding group, drug group and ovariectomized model group were redued,there were significantly different (P<0.05). Among them, weight coefficient of the hypothalamus of sham groupwas the highest, ovariectomized model group was the lowest.c. Adrenal weight index:Comparison with drug group, the adrenal weight of embedding group was decreased, there was no significantly different (P>0.05). Comparison with sham group, the adrenal weight of ovariectomized model group was reduced, there was very significant differences (P<0.01); Among them, the adrenal weight of normal group was the highest, ovariectomized model group was the lowest.d. The uterus index:Compared with ovariectomized model group:the uterus weight coefficient of the embedding group were reduced significant (P<0.05);Compared with drug group:the uterus weight coefficient of the embedding group were reduced significant (P<0.05); Compared with the sham group:the uterus weight coefficient of the embedding group, drug group and ovariectomized model group were reduced very significant (P<0.01); Among them, the uterus weight coefficient of the normal group was the highest, the uterus weight coefficient of the embedding group is the lowest.e. Weight coefficient of fat:Compared with ovariectomized model group:the weight coefficient of fat in embedding group, drug group, ovariectomized model group and normal group was reduced significant (P<0.05); Compared with drug group:fat weight of embedding group was reduced, but there was not significantly different (P>0.05); Among them, fat weight of normal group was the highest, the embedding group was the lowest.(3) Aromatase P450 mRNA expression:Compared with ovariectomized model group: the aromatase P450 mRNA expression evel of the embedding group, the drug group was increased obviously (P<0.05); Compared with drug group, the aromatase P450 mRNA expression evel of the embedding group was increased, but there was not significantly different (P>0.05); Compared with sham group:the aromatase P450 mRNA expression evel of the embedding group and ovariectomized model group were increased, but there was not significantly different (P>0.05); Among them, the aromatase P450 mRNA expression evel of the embedding group was the highest, the ovariectomized model group was the lowest.Conclusions(1) The weight of ovariectomized rat was marked increased, the therapy of catgut implementation can curb the increase weight of ovariectomized rat. (2) The aromatase P450 mRNA expression evel was increased after ovariectomized Catgut implementation can affect the aromatase P450 mRNA expression to regulate the function of estrogen. (3) In rats after castration may cause the uterus, the hypothalamus gland and other organs of the aging, degradation, reducing the organ coefficient; Acupoint catgut embedding therapy Can increase the ovariectomized rat uterus, the organ coefficient of the hypothalamus, to improve the organ's aging, the degradation of the situation. |
PDF Full Text Request