| Objective:The aim of this study was to compare concentrations of Ca,Fe,Ni,Cu,As,Pb in blood serum and tissues of 39 gasric cancer patients and 48 breast patients (15 malignant,14 fibroadenoma and 19 hyperplasia) with 66 healthy donors. The expectation of this study is to reveal mutual correlations of distribution of trace metals in the tissues and serum of cancer patient, and variability of trace metals in the serum of patients and healthy donors.Methods:The blood serum and pathological tissues of gastric cancer patients, breast patients were collected from in-patients in the First Hospital of Lanzhou University. And these samples were pretreated through nitric acid-perchloric (4:1, v/v) acid based wet-digestion. The mean concentrations of trace metals (Ca,Fe,Ni,Cu,As,Pb) in these samples were determined by the Inductively Coupled Plasma-Atomic Emission Spectrometer method (ICP-AES) and dual-channel Atomic Fluorescence Spectrometry. The results were analysised by SPSS 13.0.Results:1. Gastric cancer patients:(1) The mean concentrations of Ca, Ni, Cu and As were found to be significantly higher in the serum of gastric cancer patients compared with those of the controls (P<0.01), the mean concentrations of Pb was higher than those of the controls but no significant differences. Fe was not observed in the serum of controls and gastric cancer patients. (2) The average concentrations of Ca,Ni, Cu, As, Pb and Fe were lower in the cancer tissues than those of surrounding non-tumour tissues. Among them, the mean concentration of Ca was significant differences between cancer tissues and surrounding non-tumour tissues (P<0.01), the mean concentrations of Fe was difference between cancer tissues and surrounding non-tumour tissues (P<0.05). The rest of trace metals were no significant differences (P>0.05).2. Breast disease patients:2.1.The distribution of trace metals in the serum of breast disease patients and controls:(1) The mean levels of Ca, Cu. Ni. As were found to be significant higher in the serum of breast cancer patients compared with those of the controls (P<0.01), but the mean concentration of Pb was lower than those of the controls (P>0.05). (2)The mean concentrations of Ca, Cu, Ni and As were higher in the serum of fibroadenoma patients compared with those of controls but no significant differences (P>0.05), the mean concentration of Pb was lower than those of the controls but no significant differences. (3)The mean concentrations of Ca, Cu, Ni and As were found to be higher in the serum of hyperplasia patients compared with those of the controls, but the mean concentration of Pb was lower. Among them, the mean concentrations of As,Pb were significant differences between hyperplasia patients than those of controls (P<0.01), the rest of trace metals were no significant differences (P>0.05).2.2 The distribution of trace metals in the serum of breast patients:(1) The mean concentrations of Ca, Cu, Ni and As were higher in the serum of breast cancer patients compared with those of fibroadenoma patients, the mean concentration of Pb was lower. Among them, the mean concentrations of Ni and Cu were significant differences (P<0.01) and the mean concentration of Ca was differences (P<0.05) between breast cancer and fibroadenoma patients, the mean concentrations of As and Pb were no differences (P>0.05). (2) The mean concentrations of Ca, Ni and Cu were higher in the serum of breast cancer patients compared with those of hyperplasia patients, but the mean concentrations of As and Pb were lower. Among them, the mean concentrations of Ni, Cu and Pb were significant differences (P<0.01) and the mean concentration of Ca was differences (P<0.05) between breast cancer patients and hyperplasia patients, the mean concentration of As was no differences (P>0.05). (3) The mean concentrations of Ca, Cu, As and Pb were lower in the serum of fibroadenoma patients compared with those of hyperplasia patients. However, the mean concentration of Ni was higher. Among them, the mean concentration of As and Pb were significant differences (P<0.01), the rest of trace metals were no significant differences.2.3 The distribution of trace metals in the tissues of breast patients:(1) The mean concentrations of Ca, Fe, Ni, As and Pb were higher in the breast cancer tissues than those of fibroadenoma tissues, but the mean concentration of Cu was lower. Among them, the mean concentrations of Pb was significant differences between breast cancer tissues than those of fibroadenoma tissues (P<0.05), the concentration of other metals were no significant differences. (2) The mean concentrations of Ca, As and Pb were higher in the breast cancer tissues than those of hyperplasia tissues, the mean concentrations of Fe, Ni and Cu were lower in the breast cancer tissues than those of hyperplasia tissues, the mean concentrations of Fe was significant differences between breast cancer tissues and hyperplasia tissues (P<0.01), the mean concentrations of Pb was differences (P<0.05), the concentration of other metals were no significant differences. (3) The mean concentrations of Ca, Fe, Ni, As and Pb were lower in the breast fibroadenoma tissues than those of hyperplasia tissues. However, the mean concentration of Cu was higher. Among them, the mean concentrations of Fe was significant differences between the breast fibroadenoma tissues and hyperplasia tissues (P<0.01), the concentration of other metals were no significant differences.Conclusion:(1) The mean concentrations of Ca, Ni, Cu and As were higher in the serum of gastric cancer patients, breast cancer patients, breast fibroadenoma patients, breast hyperplasia patients compared with those of the controls; the mean concentrations of Ca, Ni, Cu and As were found to be significant higher in the serum of gastric cancer patients, breast cancer compared with those of the controls; the mean concentration of As was significant differences in the serum of hyperplasia patients than those of the controls. The level of Pb was lower in the breast patients than those of control. However, the level of Pb was higher in the gastric cancer patients than control. The concentration of Pb was altitude accumulation in breast cancer patients. (2) The mean concentrations of Ca, Cu, Ni, As, Pb and Fe were lower in the cancer tissues than surrounding non-tumour tissues, but the correlation of trace metals were stronger in the cancer tissues than those of surrounding non-tumour tissues. (3) The mean concentrations of Ca, Cu and Ni were found to be significantly higher in the serum of breast cancer patients compared with those of fibroadenoma and hyperplasia patients. Furthermore, the correlations of trace metals were stronger in the breast cancer patients than those in the fibroadenoma and hyperplasia patients. The change of concentrations of trace metals may be closely related to the generation of gastric and breast pathological changes. However, the correlations between them need further investigate. |
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