Comparative Proteomics Analysis From The Serum In Retinoic Acid-induced Cleft Palate In Mice

Objective To explore the protein changes in serum of the cleft palate disease, the animal model of the retinoic acid induced cleft palate and the comparative proteome analysis were used in this study.Methods Sixty C57BL/6J strains of mice were used in this study. For the experiment group, E10 pregnant female mice were given by gastric intubations of a single dose of all-trans retinoic acid (RA) (100 mg/kg) and the RA was dissolved in olive oil. The control group were given the equivalent volume of the carrier alone. The normal group did not give anything. Serum were collected from the pregnant mice at E14.2-DE, MALDI-TOF MS, and Western blotting were applied to analysis the protein changes in the serum of the normal, control, and RA pregnant mice groups.Results Haptoglobin B-chain and serum amyloid P-component levels in sera of RA pregnant mice were significantly higher than those from normal or control subjects.Volum% for Haptoglobin B-chain and serum amyloid P-component were 3 and 2.5 times higher,respectively. In addition, N-glycans of haptoglobin B-chain were found differently altered expression between RA groups and normal or control groups. Each of these differences was statistically significant(p value<0.01),p value obtained by Mann-Whitney U-test.Conclusion The expression level of the protein, haptoglobinβ-chain and serum amyloid P-component, in the serum of RA pregnant mice group were significantly higher than those of the normal and control groups. Differences in structure of the N-glycans of haptoglobin B-chain were found in RA pregnant mice. It was suggested that these potential functional proteins provide insights into the pathogenetic mechanism of cleft palate caused by excess RA remains.