A Study On Biodegradable PLGA Microspheres As A Delivery System For Vaccine

Objective Microspheres were prepared by double--emulsion solvent evaporation method, using BSA as modle protein and PLGA as controlled-release carrier. To discover preparation of microspheres and morphology and encapsulation efficiency. Finally, using high encapsulation efficiency was prepared Human papilloma virus (HPV) E7 protein (PGLA) microspheres, to immunize C57BL/6 mouse with the subcutaneous administration To evaluate the immune response of PLGA-MS.Methods Using two kinds of microspheres were prepared by Emulsion solvent evaporation method, and with CBB Methods to measurement of protein content, and invitro release of microspheres. HPV E7 protein was used an antign absorb different adjuvant conclude aluminum hydroxide hydrate [Al(OH)3], Dimethyl dioctadecylammonium (DDA) and poly(lactic-co-glycolic) acid (PLGA) microspheres to immunize C57BL/6 mouse with the subcutaneous administration. Antibody levels in serum were detected by indirect ELISA. and To evaluate the immune response of PLGA-MS used as carrier to carry the vaccinum.Results (1) Using Emulsion solvent evaporation method to preparation BSA-PLGA, we can see the Microspheres were smooth surface, spherical particles, diameter 5-10um. Encapsulation efficiency using extraction hydrolysis were 39.58% (2) PLGA Microspheres were surface of Spherical, uniform size and diameter 1-2um, surface associated BAS protein were 56.3% (3) PLGA Microspheres adsorption of human papilloma virus (HPV) E7 protein to preparation HPV16-E7-PLGA Microspheres. With he electron microscope, We can see the absorb of E7 protein the surface of different,some into a cord-like or Irregular, no adsorption of proteins is still spherical. The encapsulation efficiency of microspheres were 21.4%, immune C57BL/6 mice with Subcutaneous immunization mean used an antign absorb different adjuvant conclude aluminum hydroxide hydrate [Al(OH)3], Dimethyl dioctadecylammonium (DDA) and poly(lactic-co-glycolic) acid (PLGA) microspheres,6 weeks later, IgG1 antibody levels in C57BL/6 mouse injected by E7 protein of HPV-loaded PLGA microsphere was higher than those injected with E7 protein of HPV puls Al(OH)3 and DDA (the midpointtiters were 3805,1270,2262, respectively); IgG2b antibody levels was significantly higher than that of Al(OH)3 and slightly less than that of DDA (the midpointtiters were 1131,475,2653 respectively); In terms of the IgG2b antibody levels, It is higher than that of Al(OH)3 and DDA (the midpointtiters were 150,36,106 respectively).Conclusions (1) Using two kinds of microspheres were prepared by Emulsion solvent evaporation method, we can see the Microspheres were smooth surface, spherical particles. Microspheres adsorbed BSA protein rate are higher than PLGA-BSA Microspheres. (2) Biodegradable PLGA microsphere was an effective system for conteolled delivery of vaccines. For the treatment of cervical cancer vaccine it can to provide a theoretical basis for exploring the development of better vaccines.