The Effect Of Hot-Compress Medicine On Skin Thickness And Type â…  Collagen About Experimental Animal Model Of Scleroderma

Objective: to observe the changes of thickness and collagen type I of experimental animal skin by "hot-compress medicine", and provide experimental basis data about "hot-compress medicine" treatment to anti-fibrosis of scleroderma.Methods: Taking the method of scleroderma Yamamoto, 60 BALB/c mice randomly divided into two groups, one group is blank group includes 10 mice, other group is model group includes 50 mice .Each one's back fur was shaved away and the size of them are 1.5 cm×1.5cm ,then injecting BLM injection in 200ug/ml with 4 1/2 needles in the central area within skin to the model group, 0.1 ml/time; by the same method, injecting PBS injection to the blank group , 0.1 ml/time,1time/day. Both groups have been injected for 5 weeks continued. Hot-compress medicine simulation method, the clinical medication with "hot-compress medicine" paste soaked or smear lesions, the method such as scleroderma "hot-compress medicine" powder (300g/bag) added water 3000, 1500, 750ml mixing boil, make 10%, 20%, 40% medicine paste. Will model group 50 mice then divided into small concentration group, the low concentration group, the large concentration group, the model group, the western medicine group, each group contain 10 mice, the corresponding only skin lesions in back outside to 10%, 20%, 40% hot-compress medicine paste, model group and western medicine group without drugs with heparin sodium ointment, 1 times per day, continuous one month. One time per day for 5 weeks continued. After fasting, and before the drug by water, the next day reminiscent of the spinal cord, take back death dislocated method by skin tissue 10% formalin liquid fixed. Take skin tissues dehydration, paraffin embedding, HE after dyeing, using analysis software analysis mice skin thickness. Using immunohistochemistry technical inspection group typeⅠcollagen in mice skin tissue protein expression through relevant software analysis, immunohistochemical index. Through the observation of scleroderma medicinal drug heat before and after a mouse model of general morphology and skin lesions, and the change in the thickness of the material of organ fibrosis extracellular matrix the main ingredients (typeⅠcollagen) content changes, clear hot compress drugs on the mouse model scleroderma skin fibrosis treatment function.Results: generally display: dermal thickness: The analysis between blank group and each groups, P > 0.05, the result hasn't statistical significance, it means skin thickness no change. Pairwise analysis between groups about typeⅠcollagen average grayscale value: the result between blank group and the rest group is different, P < 0.05; Model group and low concentration group, no difference, P > 0.05; the result among model group, moderate concentration group and high concentration group have obvious different, P < 0.05;It is different between moderate concentrations group and high concentration and the effect of high concentration group is more effective than moderate concentrations group, P > 0.05.Conclusion: 1. "hot-compress medicine" topical scleroderma skin thickening has no obvious effect of improvement. 2. "hot-compress medicine" external use can reduce typeⅠcollagen protein expression, can improve scleroderma, and curative effect with lesions fibrosis higher concentration enhanced.