The Study Of Syndrome Differences In Methylation Egulation Based On Twins

Objective:1. To Discusses the rationality and feasibility of using the theory of twin study method.2. Use some ways of epigentics to discusses the sydorme study in twin method.3. To screen methylated differential gene expression of kidney deficiency at the gene level in diabetes of twins.Method:1. theory study method:to begin with twins as natural material and the particularity of TCM syndrome,and study the history,concept and content of twin study method,point out twin study method is develop of TCM syndrome. And epigenetics to combine with twin study method and syndrome establish new ways of syndrome study.2. Epidemiology investigation:to find out the sydrome regular of new born, contrast with twins and single both. And clinical investigations with scaling tables on four diagnosis and kidney-deficiency.3.empirical study:Screen differential gene expression of the diabetes kidney-deficiency by DNA methylation chip.Results:1. TCM syndrome study with twins methods reveal the essence, and profit in diagnosis precaution and treatment.2. Epidemiology investigation show that twins is the best material of syndrome.3. The ways of epigenetics have the distinctive competitive advantage.4. The key Pathogenesis of diabetes kidney-deficiency is glycometabolism and immunologic function disorder.5. MMP-9,UGDH and GART exceptional closely related to diagnosis.6.The disagreements gene of methylation regulation closely related to kidney-deficiency.Conclusion:Twins especially homozygotic twin have same genetic background is the best material in human study, twins method as a research ways hold more and more attention.. TCM sydorme have had obtain mass data from several angle, but still can not assurance the essential of syndrome, which need to introduce the theory and means of epigenetics research.Through investigate of a pair of diabetes twins syndrome, show the improntance of kidney-deficiency in type 2 diabetes. The epigenetics as the hot point of life science were the ligament between genotype and phenotypic. Use the new ways to analysis the syndrome essential show the positive influence of epigenetics control. some candidate differentially expressed in type 2 diabetes with kidney deficiency were screende by DNA methylation chip experiment, provide thred and method in syndrome family.