The Expression And The Clinical Significance Of MIF And TNF-α Protein In Human Hepatocellular Carcinoma

Objective: To study the expression of macrophage migration inhibitory factors (MIF) and tumor necrosis factor-α(TNF-α) protein in human hepatocellular carcinoma(HCC) and the relationship with its clinical features. Methods: The expression of MIF, TNF-αin 60 cases with HCC, 20 cases with adjacent normal tissues as control group were detected by immunohistochemical technique. All patients were not treatment by chemotherapy, radiotherapy or other anti-cancer therapy before surgical operation. Results: 60 cases of liver cancer tissues, MIF protein expression positive, 46 cases positive expression rate for 76.6% (46/60); TNF-αprotein expression positive in 12 cases, masculine expression rate to 20% (12/60). In 20 cases of cancer in normal tissue adjacent, MIF and TNF-αprotein expression positive separately for 6 patients (30.0%) and 17 cases (85.0%). Carcinoma in normal tissue adjacent MIF and TNF-αprotein expression significantly higher than cancerous tissue, statistics shows that there is difference (P < 0.05). Clew liver tissue MIF and TNF-αprotein expression, affecting the normal protein expression level, presumably by certain by MIF signaling pathways promotes tumorigenesis and transfer that TNF-αmay signal transduction pathways through induced cytokines, maintain the normal tissue cell apoptosis, thus inhibiting the program tumor development, both the expression level of liver tumors aberrations development plays a role, both in tumor growth process may exist certain correlation. But the mechanisms of signalling pathways related experts at home and abroad, the specific research thoroughly not relevant mechanism needs to be studied further.Conclusions (1) MIF in adjacent normal tissues were significantly lower than tumor tissue, on the contrary in the adjacent normal tissue TNF-αprotein expression was significantly higher than in tumor tissue of HCC, MIF-induced signaling pathway may play a important role in the formation of HCC, TNF-αinduced signaling pathway may inhibit the pathogenesis of HCC. (2) MIF is highly expressed and low expression of TNF-αprotein in HCC, and in some way to promote and inhibit the occurrence of HCC development and metastasis, suggesting that MIF and activation and blocked TNF-αinduced signaling pathway may control development of HCC ,and maybe provide the molecular basis for targeted therapy of HCC.