Effect Of Central Vein Catheters At Different Times On Focal Vessel Wall In New Zealand White Rabbits

Objective: To investigate the changes of EC,vessel wall and CRP after Central Vein Catheters at different times and determine a proper retention time.Methods: Models of central vein catheterization were established in 42 rabbits. And the animals were randomized into seven phases of six rabbits each according to catheter indwelling times of 0 d,1week,2week,3week,4week,5week and 6week. Immunoturbidimetry was used to detect the CRP and FIB of Blood specimens. After the animals were sacrificed, the catheter, access vein were dissected. The catheters were cultivated, Histological evaluation was performed by using standard light microscopy on Hematoxylin-eosin stained specimens. Scanning electron microscopy was used to observe the damage of endothelial cells.Results:①The models were constructed Successfully.②All catheters cultivated:(-)③The endothelia damage were more severe at 4week than 3week (p<0.05)④Inflammation reaction were found at 4week, thrombus and calcification at 5week and 6week⑤The expression of CRP and FIB started the day after intubations and reached the maximum at 4week than 3week(P<0.05).Conclusion:①The method of central vein catheters in New Zealand white Rabbits through external jugular is simple and can offers a platform for further experiments.②With the central venous catheter extended ,4-5w cycle of endothelial damage and inflammatory reaction that may lead to irreversible tissue pathological changes caused by remodeling③After CVC ,The damage of EC and inflammation reaction are severe at 4week .