The Expression Of Trx System In The Kidney Of Type 2 Diabetic Rats And The Effects Of Probucol

Objective: To observe the expression of thioredoxin and thioredoxin- interacting protein in the kidney of the type 2 diabetic rats induced by streptozotocin(STZ). After 8 weeks of probucol treatment, we observe the effect of probucol treatment on thioredoxin system .Methods: 30 male SD rats of 7 weeks old were randomly divided into the control group(C,n=10),the diabetes group(D,n=10) and the diabetes probucol treatment group(P,n=10) after adaptive feeding for 4 weeks. C was fed with regular diets, D and P was fed with the diets enriched with high-fat and high-sucrose. 4 weeks later, D and P were injected intraperitoneally with streptozotocin(STZ)diluted at a dose of 30mg/kg body weight, C were injected intraperitoneally with the equivalent volume of citrate buffer. D and P was still given the diets enriched with high-fat and high-sucrose. At the same time P group were orally administered probucol. After 8 weeks, measured the body weight,24h microalbuminuria (ALB),blood urea nitrogen ( BUN ),Creatinine (Cr ),the fasting plasma glucose (FPG),fasting insuline (FINS),Malondialdehyde(MDA),Superoxide dismutase(SOD) and Catalase(CAT).To observe the expression of Trx and Txnip in the kidney of three groups by immunohistochemistric method,RT-PCR and Western blot.Results: Compared with the control group, the expression of Txnip,24h ALB,FPG,BUN,Cr and MDA of P and D group were significantly increased (P<0.05), however the markers of P group were significantly decreased compared with D group(P<0.05). The body weight,Trx,SOD and CAT of P and D group were much lower than C group(P<0.05), but the markers of P group were apparently higher than D group(P<0.05).Conclusions: Under high glucose, the activities of oxidative stress were enhanced. The intracellular and extracellular redox balance such as Trx system was destroyed, which is related to the renal damage. After the probucol treatment at the early time, probucol can increase Trx expression and reduce Txnip expression in kidney, which is correlated with its role of protecting the renal.