Osteogenesis Of Adenovirus Mediated BMP2-15 Inducing Multipotential Differential Stem Cell C2C12

Objective: BMP2-15 recombinant adenovirus were constructed by ping pang multi-stages infection with adenovirus package cells HEK293, then virus titer and function were tested and verified which established the foundation of further osteogenesis study based on BMP2-15.Method: BMP2-15 recombinant adenovirus were constructed by ping pang multi-stages infection and cells frozen/dissolved. Virus titer was tested with GFP marked comparison. Expressional function of BMP2-15 were verified with RT-PCR.Results: (1) High titer BMP2-15 recombinant adenovirus were harvested . (2) After effective infection of C2C12 with Ad5-BMP2-15, Expressional function of BMP2-15 were verified with RT-PCR. Conclusion: We can construct highly expressional adenovirus mediated BMP2-15, and the target gene BMPs efficiently expressed after infected HEK293 and C2C12. nPART TWO OSTEOGENESIS OF ADENOVIRUS MEDIATED BMP2-15 INDUCING MULTIPOTENTIAL DIFFERENTIAL STEM CELL C2C12Objective: C2C12 cells were infected with adenovirus mediated BMP2-15 and bone formation was induced. The differences of osteogensis induced by BMPs were studied in vitro and vivo.Methods: (1) Multipotential differential stem cells C2C12 were infected with Ad5-BMP2-15, then the differences abilities of osteogensis were analyzed by ALP activity test. The most efficacy BMP were selected for further study. (2) C2C12 cells which induced with the favorite BMP subcu-injected into the nude mice ,and the differences of bone formation were compared in vivo.Results: (1) The favorite genes BMP2,4,6,7,9 were selected for further research. (2) Osteogenesis induced by BMP2,4,6,7,9 were tested in vitro and vivo, and we found BMP9 has the most efficient ability of bone formation except traditional BMP2 and BMP7.Conclusion: We infected C2C12 cells with adenovirus mediated BMP2-15, and tested the ability of bone formation induced by these BMPs with ALP activity test. The favorite genes BMP2,4,6,7,9 were selected which all have the potential of osteogenesis induction. We hypothesize that BMP9 has the most efficient ability of bone formation except traditional BMP2 and BMP7.