Effect Of Microenvironment Of Tongue Squamous Cell Carcinoma On The Growth And Differentiation Of Bone Marrow-Derived Dendritic Cell

Objective: To investigate the effects of different phase microenvironment of tongue squamous cell carcinoma on the growth and differentiation of bone marrow-derived dendritic cell.Methods: Marrow precursor cell were gained from thighbone of C57BL/6 ,then cultured in RPMI1640 medium containing rmGM-CSF and rmIL-4 +either different does of cs-tca8113 or not. Control groups were cultured without any cs-tca8113. The morphology of DCs were observed under optical microscope on 3th and 5th Day. At day 6 of culture ,suspension cell of groups were collected ,half of the cells was calculated by trypan blue ,the phenotypes of cells were analyzed by flow cytometry ; the other part of cells were stimulated by LPS for 24h, These DCs'phenotype of cells was analyzed by flow cytometry,their ability to stimulate T lymphocyte proliferation were compared by mixed lymphocyte reaction in vitro.Results: 1.The cells cultured in tumor microenvironment appear typical morphotype and phenotype.2.Microenvironment of low dose cs-tca8113 stimulated DCs to mature ,microenvironment of medium dose cs-tca8113 do not inhibit the maturation of DCs ,but promote bone marrow precursor to differentiate into imDC ; Microenvironment of high dose cs-tca8113 inhibit DCs to differentiate and mature .Conclusion: Bone marrow precursor cells can be successfully induced into DCs with typical characteristics in the microenvironment of Tca8113,the multiple effects of tca8113 microenvironment on the growth and differentiation of DCs closely relate to the concentration of cs-tca8113.