Induction Of Apoptosis In TF-1 Cell Line By Bortezomib And Its Influence On The Expression Of SALL4 Gene

Objective: To investigate the effect of bortezomib on proliferation and apoptosis of human erythroleukemia cells TF-1, and its influence on the expression of Sall4 mRNA. Methods: (1)The level of cell proliferation was analyzed by MTT assay. (2)Morphological changes were observed by microscope.(3)Morphological changes were observed by electron microscope.(4)Flow cytometry with Annexin-V staining was used to analyze cell apoptosis rate (5)Flow cytometry was used to analyze and cell cycle distribution.(6)The level of the expression of Sall4 mRNA was detected by RT-PCR. Results: (1)Bortezomib inhibited TF-1 cells in a time-and-does dependent manner. The IC50 at 24 and 48 hours were 52 nmol/L and 27 nmol/L respectively.(2) Under microscope, it could be observed that the density of cells declined while cell fragments increased.(3)Moreover, under electron microscope, there appeared some apoptotic bodies.(4)Bortezomib could induce apoptosis of TF-1 cells in a dose-dependent manner.(5)At the same time, it caused an increase in G2/M phase and a marked decrease in G0/G1 and S phases in a time-dependent manner.(6)It was found by RT-PCR that the level of Sall4 mRNA was down-regulated with the treatment of bortemomib. Conclusion: Bortezomib can cause apoptosis of TF-1 cells and down-regulate the expression of Sall4 mRNA in a time-and-does dependent manner.