Effects Of Perinatal Malnutrtition On ACHE And CHAT Of Hippocampus In The Male Rat Offspring

Objective: To explore the effects of perinatal malnutrtition on AchE and ChAT of hippocampus in the male rat offspring in the 21days after birth. So that to study the effect of perinatal malnutrition on the onset of dementia.Methods: Eight Sprague-Dawley rats,with 14 days'pregnant, were purchased and housed individually. And randomly divided the rats into two groups: food-restricted group(FR) ,which received 50% of the food-intake of control mothers(CM) , which were not restricted with food ,from pregant day 14 to postnatal day 21 (refer to Coupe B's feed standard). We made the mothers feeded the same number of pups factitiously(only 8 pups kept every nests, if more than 8, discassed the others). We weight the rats pups every day and noted down the information in ervery morning 9 o 'clock from the first day to the 21st day postnatal, to compare the difference between the two groups.And at the end of lactation (the 21st day postnatal) , the male offspring were humanely killed and the brain were obtained for measurement.To circumvent this study, we used the HE staining to observe the hippocampus neurons'form and immunohistochemical staining to assay the optical density of positive ChAT and positive AchE cells in the hippocampal CA1 and the CA3 layer,and determining the levels of mRNA of AchE and ChAT in Real-time PCR.Results: There was no significant difference in the average birth weight of pups between the model and control groups(6.28±0.05 vs 6.12±0.40, P>0.05). However, in the 3,7,14 and 21 day postnatal,compaired with the weight of pups in the control group, the FR offspring was obviously lighter in all different experimental time points(all P<0.05) .The immunohistochemical staining results for ChAT and AchE: The optical density of positive ChAT cells in the hippocampal CA3 layer of FR male offsping decreased from 0.29±0.02 in control group to 0.23±0.02 in model group, and the ChAT expression significantly changed in the CA3 layer in model group(P<0.05), while in the CA1 layer ChAT expression was no difference comparing with control group(P>0.05). In both the CA3 and CA1 layers, There was also no significant difference in the optical density of positive AchE cells in model and control groups(all P>0.05).The RT-PCR results for ChAT and AchE: Compared with control group(ChAT: 0.75±0.11; AchE: 0.34±0.07), The model group in both ChAT and AchE mRNA expression significantly decreased(ChAT: 0.61±0.14 ; AchE: 0.29±0.06; all P<0.05).Conclusion: Perinatal malnutrtion could prohibit the development of central cholinergic system among male rats offspring,which would affect the lenarning and memory ability after they grown up.So we guess that the perinatal undernutrition would be relation to the onset of demential.