Research On The Mechanism Of Shiraia Bambusicola Intervening In TNFα Signaling Network

Objective:To verify the anti-inflammatory and antineoplastic effectiveness of Shiraia bambusicola and its possible mechanism, we bring in a TNFa induced cell line L929 as subject and a model to observe the TNFa signaling pathway, we detect the effect of Shiraia extracts on the model cell and detect the variation of some key roles in the pathway to analyze the possible mechanism of Shiraia extracts on treating inflammation and tumor. The results obtained from this research may provide a experimental and theoretical basis on the careful development of this traditional herb medicine.Methods:(1) Growth inhibition of model cell was measured by SRB assay; (2) Morphological changes of the cells were observed under the invert microscope and fluorescence microscope; (3) Apoptosis rate of model cell and cell cycle condition was analysed by FCM; (4) Western blotting was applied to detect the expression of NF-κB P65 subunit,COX-2 and BCL-2.Results:(1) Shiraia extracts could reduce the inhibition effect of TNFa in L929 on certain concentration, however when the concentration of Shiraia extracts up to 30μg/ml it enhancing such inhibition effect; (2) Shiraia extracts can decrease the S phase arrest of L929 cell which induced by TNFa; (3) Shiraia extracts slightly inhibit the expression of NF-κB P65 protein induced by TNFa; (4) Shiraia extracts remarkably inhibit the expression of COX-2 protein induced by TNFa;(5) Shiraia extracts stimulate the expression of BCL-2 on certain concentration.Conclusion:(1) Shiraia bambusicola contains different substances with antagonistic biological activity,which give Shiraia a two-direct adjustment features. (2) In a certain range of concentration, Shiraia influence the TNFa signaling network through the COX-2, BCL-2 signaling pathways but has no significant effect on NF-κB signaling pathway.