The Study Of Association Between Clock Gene T3111C And T257G Polymorphisms And Sleep Epilepsy In A Hunan Han Population

Objective:The objective of the present study is to investigate the association between the Clock T3111C and T257G gene polymorphisms and sleep epilepsy patients in Han population of Hunan province.Methods:311 subjects with epilepsy and 300 sex--and age--matched healthy controls were enrolled in the study.Genomic DNAs were extracted from peripheral blood leucocytes bu phenol-chloroform methods.The Clock T3111C and T257G polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)Results:1. Two genotypes(TT and TC) were detected in Clock T3111C. The frequency of Clock site T3111C genotypes in all of the people is 86.09%(TT),13.91%(TC),0.0%(CC), T allele gene frequency is 93.04% and C allele gene frequency is 6.96%. There is insignificant difference of genotype and gene distribution of Clock gene T3111C polymorphism in Sleep epilepsy group,Aperiodic group,Awakening epilepsy group and Control group. 2. Two genotypes(TT and TG) were detected in Clock T3111C. The frequency of Clock site T257G genotypes in all of the people is 85.92%(TT),14.08%(TG),0.0%(GG), T allele gene frequency is 92.96% and G allele gene frequency is 7.04%. There is insignificant difference of genotype and gene distribution of Clock gene T257G polymorphisms in Sleep epilepsy group,Aperiodic epilepsy group,Awakening epilepsy group and Control group.3. There is an almost complete correspondence (complete linkage disequilibrium) of bases between the positions 257 and 3111.Conclusion:Clock gene T3111C and T257G polymorphisms are not associated with sleep epilepsy in Han population of Hunan province.