The Research Of Tetramethylprazine To Prevent Vascular Restenosis In Vein Grafts

Objective To study the Effects of Tetramethylprazine(TMP) on the vascular intima hyperplasia and apoptosisi of smooth muscle cell in rat venous bypass grafting.Methods Sprague-Dawley rats were underwent interposition bypass grafting of the common carotid artery via the ipsilateral external jugular vein. The rats were divided into three groups: sham group, matched group and TMP group. Drugs were administered through lavage before two weeks of the operations. TMP grpup were offered TMP 100mg/Kg, and the other two groups were given equivalent distilled water. All were established the animal models, and used Doppler ultrasonography to inspect them whether they were unostructed or not three days, seven days and ten days later, respectively. The grafts were harvested for histochemical staining and the level of proliferating cell muclear antigen (PCNA) was detected by immunohistochemistry.Results The grafts were verified whether they were unobstructed or not by Doppler ultrasonography. The Mathed group's vein grafts smooth muscle cells developed more significantly proliferate than the TMP group's, resulting in stenosis of the vessel. TMP group's neointimal hyperplasia was restrained, and the neointimal areas and expression of PCNA in the TMP group were significantly lower than the matched group(P<0.05), however, the percentage of apoptosis was significantly higher than the sham group and the matched group(P<0.05).Conclusions With the treatment for TMP, the neointimal formation and smooth muscle cell proliferation can be inhibited in the vein graft, showing the favourable prospects in preventing and curing vascular restenosis.