| Background and PurposeAge-related macular degeneration is a common visual impaired disease in aging population. It is the leading cause of vision impairment in western countries which influences the quality of life of patients severely. The pathogenesis of AMD is not clear. Incidence of AMD is rising rapidly as the population becomes aging in China Epidemiological studies of AMD are quite few in China so far. We did an association study to investigate the risk factors of AMD from aspects of environment and genetics.MethodsWe included 89 AMD cases and 143 controls in this study. The cases were AMD patients diagnosed in department of out-patient or in-patient. The controls were frequency matched, randomly sampled from non-AMD patients in the same hospital.. A uniform questionnaire was used for each subject and venous blood was collected from each participant. Each of the subjects signed an informed consent, The Real-time PCR (polymerase chain reaction) and TaqMan assay were used to analyze genotypes of all selected SNPs. Univariate, multivariate and stepwise logistic regression models were used to explore and screening the important risk factors of AMD, odds ratio (OR) and 95% interval confidence (95%CI) were estimated accordingly. Hardy-Weinberg Equilibrium (HWE), Linkage disequilibrium (LD) and Haplotye analysis were performed using SHESIS. Other statistical analysis were done by SAS 9.13 and RevMan 4.2.ResultsAll participants were Chinese Han people aged older than 45 years. Education, watching TV or computer frequently and diet with high fat were found associated with AMD when adjusted for gender and age. Low-level education was about 2.89 (95%CI:1.19, 7.00) times risk of AMD when compared with high-level education. Risk of high fat intake was about 5.84(95%CI:1.79, 19.07) compared with low fat intake. The distribution of rs10490924 genotype in case and control was statistical significance (P<0.05).SNP rs10490924 TT increased the risk of AMD with 2.90-fold (95%CI: 1.36, 6.21) compared to GG. The risk was 2.53-fold (95%CI:1.57, 5.55) when adjusted for age and gender. SNP rs11200638, rs10490924 and rs2230199 were in high LD (D', 0.98 and 0.99). SNP rs2230199, rs1410996, rs800292 and rs4151667 were in high LD respectively (D', 0.96, 0.98 and 1.00). A-T was the risk haplotye (P=0.003), while the haplotype A-G and C-A were protective (P=0.034 and 0.030 respectively). The combined effect of rs10490924 TT and rs800292 GG was 7.24 (95%CI: 2.02, 25.96).ConclusionsIn our study, high fat intake might increase the risk of AMD. Education is an important social factor for AMD. SNP rs10490924 variant is a risk factor for AMD. There may be gene-gene interaction between LOC387715 and CFH, while CFH, CFB, C2 and C3 showed no associations with AMD. |
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