Study On Dielectric Spectroscopy Of Erythrocytes In Suspension From Patients With Iron Deficiency Anemia

Objective Establishing analysis methods and parameters of a mathematical model, with Cole-Cole equations, comparing the disparity between normal and anemia blood by impedance measurement in dielectric spectrum. We have found a new technique which can detect the blood of anemia patients in rapid and accuracy way. Make sure that the effects on anemia of electrophysiology properties in blood and cells.Methods To collect the blood from normal and anemia patients, measuring the blood by impedance analyzer which type is Agilent 4294A, getting the parameters, then concerting the capacitance C and conductance G to permittivityεand conductivityκ, analyzing the experiment data which have converted. Extracting the characteristic parameters, after having compared with the differences between normal and anemia group . With the significance testing to differentiate the characteristic parameters between normal and anemia group. Then the parameters of Cole-Cole mathematical, which were established by the fitting residual analysis of parameters in theoretical and experiment. Finally, discussing the electrophysiology mechanisms of anemia.Result(1) In normal human blood dielectric constant drops accompanied by the increases of frequency, while a significant change happens in the slope of conductivity, which leads an accelerate increasing; when the frequency approaching 10⁵Hz and 10⁷～108Hz, there are two plateaus. The first characteristic frequency of normal human blood f_C1=0.73MHz, and the second characteristic frequency f_C2= 2.08MHz; Max of loss tangentΔtgδ_max=2.76.(2) In anemia blood patient we can observe the cell dielectric dispersion too , as the normal group there are also two plateaus in both dielectric constant and conductivity spectrum; the first characteristics frequency of anemia blood is 0.65MHz, the second characteristics reaches to 2.08MHz, and the _max of loss tangentΔtgδ_max=2.41.(3) Between frequency range 3×10⁴Hz and 2×10⁶Hz, in the curves ofε, the values of anemia group is lower than that of the normal ones, reduced by 7.69% (p<0.05) inεL, and 7.78% (p<0.05) decrease of ?ε. In the high frequency range from 2×10⁶ to 10⁸Hz,ε_h in both normal and anemia groups are almost the same, which predicated the isolation of anemia blood got down. Meanwhile the conductivity (κ) curves: the curve of anemia samples is entirely upper than the normal ones, which leads to the increase ofκ_L andκ_h by 24.38% and 6.59%. Respectively, these show the enhancement of conductive capacity in anemia blood's plasma and intracellular fluid; the curve dielectric loss factor (ε") of anemia falls and peak of dielectric loss factor (ε"_max) drops by 14.81% compared with normal samples, the first characteristic frequency decreased by 10.96% which means that the dielectric loss and frequency properties fell.(4) The characteristic dielectric response of anemia and normal blood of the Cole-Cole mathematical model, and the fitting curve residual are 4.10% and 4.67% respectively.Conclusion(1) The ability of dielectric decreases is demonstrated by the reduce ofε_L,Δε,ε" andΔtgδ_max.(2) The increase of capacity in conductivity is expressed by the addition ofκ_L,κ_h,Δκandκ".(3) The falls of frequency properties displayed in the drop of f_C1.(4) The established of Cole-Cole mathematical model provides a new technique and evaluation index on anemia electrical characteristic research, which can probably provide a useful, rapid and accuracy way for clinical diagnosis of anemia in the future.