| Objective: To investigate the protective effect of cilostazol, a phosphodiesterase 3 inhibitor, on cerebral ischemia reperfusion injury in mice; and the effect of bcl-2 expression.Method: Thirty male mice with body weight of 26-30g, and were randomly divided into three groups: group one (n=10) underwent sham operation, group two (n=10) underwent the operation of cerebral ischemia reperfusion injury but no cilostazol, group three (n=10) underwent the operation of cerebral ischemia reperfusion injury and with cilostazol (10mg/kg) ip injected 30min before operation. After reperfusion 24h killed the mice and prepare the brain tissue for TTC dyeing,HE dyeing,NOS,and western for bcl-2.Result: TTC dyeing,HE dyeing,and NOS showed that group one (n=10) underwent sham operation have no change, group two and group three were cerebral ischemia-reperfusion injury,but group three has obviously improved. The level of bcl-2 protein expression of group two was up-regulated. In comparing group two, the level of bcl-2 protein expression of group three was up-regulated(P<0.05).Conclusion: Phosphodiesterase 3 inhibitor cilostazol has the protective effect on cerebral ischemia reperfusion injury in mice and increase the expression of bcl-2. |
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