| Subjective: The purpose of this study was to definite how the carcinoma testis antigen hiwi expresses in various tumors. Methods: The hiwi mRNA expression in cell lines was detected by RT-PCR and expression of its protein in tissues was measured using immunohistochemistry. Results: Hiwi was remarkably expressed in various cancer cell lines such as Hela, MCF-7, MDAMB-231, MDA-MB-435s, A2780, and 113. Compared with that of ovarian benign lesions, hyperplasia of mammary glands, chronic cervicitis and endometrium benign lesions, the expression of hiwi was increased significantly in ovarian cancer, breast carcinoma, cervical carcinoma and endometrial cancer (P<0.01). Conclusion: These results suggested that hiwi overexpressed in diverse tumors and it played an important role in the oncogenesis. Subjiect: The aim of this study was to determine the relationship between cancer testis antigen hiwi and tumor angiogenesis. Methods: The expression of Ang-2 and Tie-2 in malignant tissues and benign tissues was measured using immune-staining.The Fisher's exact test, ANOVA SNK test and Nemenyi-Wilcoxon-Wilcox test were used to compare the differences between them. Spearman's rank correlation was used to determine whether there was a positive or negative relationship. Results: Ang-2 and Tie-2 expressed remarkably in breast cancer(BC), uterine cervical cancer(UCC), ovarian cancer(OC) and endometrial cancer (EC), and there was significant difference (P<0.01). Additionally, there was a positive relationship between hiwi and Ang-2 in UCC, BC and OC. Hiwi expression was positively related with Tie-2 in OC. Conclusion: The results show that hiwi expresses significantly in tumor vessels and is a potential target for cancer angiogenesis. |
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