Assay Of VEGF And D-dimer And Their Significance In Chronic Subdural Hematoma

Objective To investigate the role of vascular endothelial growth factor (VEGF) and D-dimer in the pathogenesis of chronic subdural hematoma(CSDH).At present,the study which in the development of chronic subdural hematoma are focused on two major areas of hyperactity in which VEGF and hematoma fluid fibrinolytic system. This experiment tests two indicators, VEGF and D-dimer. By comparing the fluid and serum in the hematoma to study whether the significant difference in the content, in order to infer whether the occurrence of chronic subdural hematoma related to the development and clinical practice of the guidance in the application.Methods The concentrations of VEGF and D-dimer in hematoma fluid and serum in patients with CSDH were detected by enzyme-1inked immunosorbent assay (ELISA) and immunoassay. Collected 30 cases, meet the test requirements of the hematoma in patients with chronic subdural hematoma fluid and blood samples, placed in 5ml test tube, the supernatant was separated after centrifugation, in strict accordance with kits require the use of enzyme-linked immunosorbent assay (ELISA) and immunoassay testing of specimens, the test result is expressed as VEGF, D-dimer results,experimental data subtotals, the use of SPSS 17.0 software for statistical data. Results The concentrations of VEGF and D-dimer in the hematoma fluid were significantly increased as compared with that in the serum (P﹤0.001). Conclusion VEGF and D-dimer may play an important role in the pathogenesis of CSDH. VEGF, D- dimer in patients with chronic subdural hematoma in the development and recurrence play an important role, so we infer that we will have a good method that we can try to inhibit the synthesis of VEGF and D-2 dimer in the treatment of chronic subdural hematoma and recurrence after surgery of the expansion process .