The Association And Mechanism Between PDGF-D, PDGFRβ Expressing In Gastric Cancer And Its Clinicopathological Features

Objective To investigate the expression of PDGF-D and PDGFRβmRNA in human gastric cancer tissues and gastric cell line SGC7901,and to explore the association between the expression of PDGF-D mRNA and clinicopathological features in gastric cancer.Methods The reverse transcription-polymerase chain reaction(RT-PCR) technique was used to examine mRNA expression of PDGF-D and PDGFRβin 58 pair specimens of gastric carcinoma and tissues adjacent carcinoma and human gastric cell line SGC7901.To explore the association between the expression of PDGF-D mRNA and clinicopathological features in gastric cancer.SGC7901 were exposed to different concentration of PDGF-D(0,20,50,100,500,2500,25000,250000pg/ml) and the growth curve was drawn by MTT.The flow cytometer (FCM) was used to analyze the distribution of cells cycle.Results The positive rates of PDGF-D mRNA and PDGFRβmRNA (98.28%,87.93%) were higher in gastric cancer tissues than (72.41%,53.45%) in tissues adjacent carcinoma respectively. While the positive rate of PDGFRβmRNA was 72.41%,which was positive correlated with PDGF-D(R=0.400,p<0.05).PDGF-D expression was correlated with lymph node metastasis and relapse(p<0.05).No correlation was found between PDGF-D and the other clinicopathological features of gastric carcinoma(p>0.05).PDGF-D expression was correlated with survival time(χ2=4.627,p=0.031.)。PDGF-D and PDGFRβmRNA were expressed in human gastric cell line SGC7901.PDGF-D could enhance SGC7901 proliferation;The distribution of cells were significantly different compared with those of the controls(p<0.05),except 500pg/ml,2500pg/ml,25000pg/ml and 250000pg/ml groups.The number of G0/G1-phase cells decreased significantly compared with those of the controls(p<0.05),except 20pg/ml groups.And S-phase cells increased except 20 pg/ml,50 pg/ml(p<0.05).Conclusion PDGF-D and PDGFRβmRNA were expressed higher in human gastric cancer. PDGF-D may play a crucial role in carcinogenesis, development, metastasis and relapse of gastric cancer. It can be used as prognostic markers of gastric cancer in clinical practice.