The Influence Of Moxifloxacin On The Expression Of INOS And Production Of NO In Vascular Endothelial Cells

The influence of Moxifloxacin on the expression of iNOS and production of NO in vascular endothelial cellsObjectives:This study was designated to study the effect of Moxifloxacin on LPS-induced expression of Inducible Nitric Oxide Synthase (iNOS) and production of Nitric Oxide (NO), and to investigate the potential mechanism associated with nuclear factorκB (NF-κB) inhibition.Methods:Vascular endothelial cells EA.Hy926 were cultured in vitro and preincubated with 5-20 mg/L MXF before 0.1mg/L LPS stimulation to assay the production of NO with Griess reagents. The expressions of iNOS mRNA and protein were detected by RT-PCR and Western blot respectively. The inhibition of NF-κB induced by MXF in EA.Hy926 cells was assessed by Western blot, the effects of pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-κB, on the production of NO and the expressions of iNOS protein were also examined.Results:MXF inhibits LPS-induced expressions of iNOS mRNA and protein and thus produces NO in dose-dependent manner in vascular endothelial cells. In the 5mg/L of MXF group, LPS stimulated EA.Hy926 cells induction of NO (26.3±3μM); when the MXF concentration reached 20mg/L, the NO decreased to (18.1±1.4)μM, with the inhibition rate of 42%. The protein of iNOS and the levels of NO were inhibited in response to LPS associated with PDTC, an inhibitor of NF-κB. The activation of NF-κB was inhibited in EA.Hy926 cells after 2 h treated with MXF, which led to the decreased p65 level in the nucleus.Conclusion:1. MXF could inhibit LPS-induced expression of iNOS and production of NO in EA.Hy926 cells. 2. MXF could inhibit NF-κB activation in EA.Hy926 cells.3. MXF inhibit LPS-induced expression of iNOS and production of NO, potentially resulting from inhibition of NF-κB.