Protective Effect Of Allicin On Human Periodontal Ligament Cell With Nicotine-induced Oxidative Damage

Objective To explore the effect of allicin on the nicotine-induced oxidative damage to PDLCs by comparing the survive rate and the levels of glutathion in the PDLCs which had been cultured with nicotine only or nicotine and allicin of series of concns.then we can supply the theorical basis of allicin's use in the therapy on periodentics.Methods(1)Periodontal ligament cells were cultured and identified in vitro by explant method.(2)Select the concentration of nicotine that can induce distinct damage to PDLCs.The MTT colorimetric method was used to detect the survival of PDLCs after 24h'trainment with DMEM which contain 0.1,0.2,0.4,0.8,1.6mg/ml respectively. (3)The MTT colorimetric method was used to detect the survival of PDLCs after 24h' trainment with DMEM which contain different concns of allicin and nicotine.(4) observe the cells after the cultivation using the microscope and take photoes.(5)the GSH in the PDLCs were determined in 1,4,8,12 and 24h respectively.Results(1)nicotine of 0.8mg/ml can damage PDLCs distinctly. So,it's choosed for the following steps.(2)The 60μg/ml allicin showed the protective effect to PDLCs.Absorptiometry of the 60μg/ml group was significantly higher than that of the nicotine group by 12%(P<0.05). There was no significant difference between the absorptiometries of the 60μg/ml allicin group and the control group (P>0.05).(3)The glutathion of the 60μg/ml allicin groups are significantly higher than those of nicotine groups at all time-points(P<0.05),the former is higher than the latter by 81% at 8h. There was no significant difference between the glutathion of the 60μg/ml group and that of the control group(P>0.05).Conclusion The allicin of 60μg/ml can protect the PDLCs from the oxidative damage induced by nicotine.