Effect Of Application Of Endothelial Progenitor Cells Combined With Estrogen Against The Restenosis After Injury Of Vascular Endothelium

BackgroundCoronary heart disease(CHD) is a kind of disease that is very harmful to people's health and a main reason for people's dealth in modern society.It has become the hot pot of preclinical and clinical medicine,but etiological factor has not been ascertained: Men usually suffer CHD at the age about forty to sixty years old,however,women appear symptoms almost after menopause.Patient with CHD usually get the risk factors,such as hyperlipemia,hypertension, diabetes mellitus, obesity,semoking and so on.Treating to CHD mainly include drugs, percutaneous coronary intervention(PCI), coronary artery bypass grafting (CABG),and gradually PCI has become one of the most effective tragedy.Though more and more new apparatus and technology have been applied to clinic,in-stent restenosis is a serious problem to the doctor and researcher of medicine.People have got some effectiveness from the application of estrogen or endothelial progenitor cells(EPC) alone,but there is no report about combination of the both.Estrogen has many actions,including accommodation of blood fat,promoting the growth and migration of endothelial cells(EC) and playing a important role in EPC.ObjectiveTo investigate the effect of application of endothelial progenitor cells combined with estrogen on the recovery of endothelium,inhibition of Neonitima proliferation and restenosis after injury of vascular endothelium. Materials and Methods1,Sixty New Zealand white rabbits were divided into five groups randomly, which were sham operation group(group-Ⅰ), control group(group-Ⅱ), estrogen treated group(group-Ⅲ), EPC treated group(group-Ⅳ), estrogen combined with EPC treated group(group-Ⅴ), twelve rabbits in each group.Three days after ovariectomy, animals in groupⅢand groupⅤwere treated with estrogen, and the other rabbits were given physiological saline instead.2,Level of estrogen was detected at different time, including before ovariectomy and 3 days and 2 weeks after ovariectomy.3,The level of blood lipids(including total cholesterol, triglyceride, high density lipoprotein choesterol and low density lipoprotein cholesterol) was also detected after hyperlipemia feed for 6 weeks.4,Mononuclear cells were isolated from peripheral blood of rabbits and cultured for implatation.5,We established the animal model of injured abdominal aorta. Animals in sham-operation group were exposed the femoral artery without injury. At the same time, we gave 2ml suspension of EPC to the rabbits in groupⅣandⅤthrough catheter and the others (exceptⅠgroup) were given physiological saline instead.6,Effectiveness of endothelium recovery, degree of cell proliferation and lumen loss were evaluated after 4 weeks.Results1,The level of estrogen in groupⅢandⅤwere obviously higher than in groupⅠ,ⅡandⅣ(P<0.01)2,The level of blood lipids,includimg total cholesterol(P<0.01), low density lipoprotein cholesterol(P<0.01) and high density lipoprotein choesterol(P<0.05) are statistically different between groupⅢ,Ⅴand groupⅠ,ⅡⅣ.3,There are also statistical significance in the degree of endothelium recovery, cell proliferation and lumen loss between groupⅤand groupⅢ,Ⅳ[(90.2±5.1) %vs(85.1±4.1)% and (83.9±6.5)%, P<0.05], [(15.67±2.98)%vs(26.02±4.18)% and (24.87±3.54)%, P<0.01],[(17.61±2.69)%vs(38.43±4.35)% and (41.15±4.45)%, P<0.01].ConclusionApplicatin of EPC combined with estrogen after injury of vascular endothelium can speed up the process of endothelial recovery, inhibit neonitima proliferation and reduce lumen loss significantly.