The Repair Of Acute Spinal Cord Injury By Bone Marrow-derived Mesenchymal Stem Cells Graft Combined With The Injection Of Monoclonal Antibody IN-1

Objective:To study the bone marrow mesenchymal stem cell transplantation combined myelin growth IN-lon the rat corticospinal tract after transection of spinal cord injury repair and significance.Methods:By density gradient centrifugation and adherent filter prepared by combining bone marrow mesenchymal stem cells, Use of clean healthy Sprague-Dawley (SD) rats were 180, weight 250-300g, male or female, were divided randomly into operated group (n= 60), BMSCs transplantation group (n= 60), BMSCs transplantation in combination with IN-1 group (n= 60), each group was in accordance with the time after surgery were randomly divided into the 1d,4d,7d,14d, 21d and 28d of six groups, each 10. All rats first line of subarachnoid catheter, then the equivalent of T8 vertebral level and marked with micro scissors cut the spinal cord dorsal 2/3 rat corticospinal tract transection injury model. Including operon control group injeaticted with normal saline through the catheter every 5μl, and BMSCs injected into each group of bone marrow mesenchymal stem cell suspension (5μl), IN-1 and BMSCs joint action group, each injection and bone marrow IN-12.5μl mesenchymal stem cell suspension 2.5μl, every day is divided into early, middle, late third injection, the longest 1 w. Taking five in each group, before surgery and after surgery 1d, 1w,2w,3w, and 4w, with BBB (Basso, Beattle, Bresnahan) score assessment of motor function, and then remove the spinal cord tissues of glial fibrillary acidic protein (glial fibrillry acidic protein, GFAP) expression changes. The remaining control group and BMSCs transplantation group, BMSCs transplantation combined IN-1 animals after injury 1d,4d,7d,14d,21d and 28d out of six time points of injured spinal cord, respectively row RT-PCR (reverse transcription-polymerase chain reaction) detection of GAP-43 gene expression data obtained by single factor analysis of variance for statistical analysis, P<0.05 significantly different results.Results:1, spinal cord injury in GAP-43 mRNA expression increased firstly and then decreased, the peak of their expression after spinal cord injury at 7d,14d,21d time point; 2, after BMSCs transplantation increases the expression of three mRNA, combined with post-IN-1 was more obvious than in each group was statistically significant (P<0.05).3, paralysis of the posterior limbs after spinal cord injury, BBB score,2w,3w and 4w point BMSCs transplantation group, BMSCs transplantation and graft IN-1 group was significantly higher than the injured control group, there was a significant difference (P <0.05); BMSCs transplantation in combination with IN-1 group than in BMSCs transplantation group, significantly different (P<0.05).4, GFAP immunohistochemistry showed that, BMSCs transplantation in combination with IN-1 group, the average gray value lower than the other two groups, there was a significant difference (P<0.05).Conclusions:(1) GFAP expression after spinal cord injury, while the IN-1, BMSCs can inhibit the expression of GFAP, which inhibit the proliferation of reactive glial cells. (2) IN-1, BMSCs upregulated GAP-43mRNA expression, and thus on axonal regeneration after spinal cord injury play a role in promoting. (3) BMSCs transplantation in combination with IN-1 can promote the survival of spinal cord injury and regeneration, and mutual synergies, better than the single application of BMSCs to promote spinal cord repair and functional recovery of rat hind limb.