Preliminary Study Of Epigenetics Gene Expressions Of Human Skin Fibroblasts During Aging

Objective Discuss the expression differences and clinical significances of a variety of epigenetics-related genes in the process of natural aging and oxidative stress-inducing senescence in human skin fibroblast cells.Approach Extract the total RNA in human skin fibroblasts from young group(under 16) and the aging group(over 55 years old) to establish the natural aging model, extract the total RNA which is sub-lethal dose H202 treated and normal control group in human skin fibroblasts to establish oxidative stress-inducing aging model, synthesize cDNA by reverse transcription, establish the standard curve of enzymes related to epigenetics gene such as EZH2, Bmi-1, JMJD3, DNMT1, MLL1 and internal standardβ-actin in the double dilution method, and then choose 10-4 concentration gradient to measure their expression level differences by real-time quantitative PCR.Results In each experimental group, when the concentration of PCR product is between (1.0×10-2)-(1.0×10-7), the above gene and internal standard gene was all amplified by a smooth curve, and the template concentration is negatively correlated with the detected fluorescence signal cycles. In the natural aging model and oxidative stress-inducing aging model, the EZH2, Bmi-1 and DNMT1 gene expression levels of the aging group were evidently lower than the young group (P<0.05), and there was no obvious difference between JMJD3 and MLL1 (P>0.05)Conclusion The contents of EZH2, Bmi-1a nd DNMT1 gene decrease gradually in the process of cell aging in human skin fibroblasts, plays a very important role during human skin fibroblast cells aging.