Influence Of Androgen Exposure In Late Trimester Of Pregnancy On Gonad Development And Reproduction Function Of Filial Female Rats

Objective:To investigate the pathogenesy of PCOS by studying the influences of androgen exposure in late trimester of pregnancy on gonad development and reproduction function of filial female rats.Methords:The number of Wistar rats is 45. It contained 30 female rats and 15 male rats. After coition pregnant Wistar rats were randomly divided into 2 groups,8 rats in each group. Group A were treated by testosterone propionate injection in a dose of 0.6mg/d on gestational d15-d20; Group B (control group) was injected with tea oil on gestational d15-d20. All rats were terminated on the 21th day. The T level of cord blood in filial female rats and their birth weight were detected; The vaginal opening and oestrous cycle of filial female rats were observed. The nipple number and weight of rats were average on postnatal day 100. The uterus and ovaries were reserved and observed.Results:â‘ The T level of cord blood in filial female rats of group A were significantly higher than that in group B(P<0.05).â‘¡The birth weight of filial female rats in group A were significantly lower than that in group B, but the weight of filial female rats on postnatal day 100 in group A were higher than that in group B(P<0.05). The filial female rats in group A had no obviously oestrous cycle which had less nipples than group B. The age of vaginal opening were unaffected by androgen exposure in late trimester of pregnancy.â‘¢Some filial female rats in group B showed hydrometra.More atretic follicles were observed in the filial female rats in group A on the postnatal day 100(P<0.05).Conclusion:Androgen exposure in late trimester of pregnancy can interfere the gonad development and reproduction function of filial female rats, such as the manifest of PCOS; Late-treated prenatal androgenization may be the early etiological factor of PCOS.