Studies On Immunological Effects Induced By Vaccine Of The E Style Chlamydia Trachomatis Recombinant Major Outer Membrane Protein And Comparition With DNA Vaccine

Even with the availability of antibiotics, Chlamydia trachomatis is the leading cause of sexually transmitted bacterial disease worldwide, with an about 100 million clinically diagnosed cases occurring annually. The majority of genital chlamydial infections are initially asymptomatic and thus not treated, and may lead to severe complications, such as pelvic inflammatory disease, ectopic pregnancy, and infertility,so cause considerable socioeconomic burden worldwide.Therefore, the need to protect against Chlamydia infections is widespread throughout the world, we also discuss specific requirements for a vaccine to protect against chlamydial STDs. Despite decades of effort, this goal has not yet been achieved with the impediments to developing a chlamydial vaccine seemingly substantial.Serovars E is the major cause of sexually transmitted infections, recent efforts have focused on developing a subunit vaccine using the major outer membrane protein (MOMP) of C. trachomatis as the antigen. The vaccine of serovars E of C.t recombinant major outer membrane protein (rMOMP) and DNA vaccine are the most prevalent researchment in the word. Therefore our laboratory constructed these two kinds of vaccine and compared the immune responses induced in mice.Objective:to express the rMOMP vaccine of C.t E serotype, evaluate and comparision the immune response and protective effect of rMOMP vaccine and DNA vaccine solo or combining in mice.Methods:BALB/c Mice were divided into nine groups. They were rMOMP plus Freund's adjutants group, solo rMOMP intramuscular group, solo DNA intramuscular group, solo rMOMP intravaginal group, solo DNA intravaginal group, DNA vaccine plus rMOMP group,PBS intramuscular negative group, PBS intravaginal negative group, and inactivated C.t positive control group. Intramuscular and intravaginal were adopted. The immune effects of C.t DNA vaccine and rMOMP vaccine were evaluated by detecting of antibodies, lymphokines, counteract ability of serum specific antibodies, and observing of DTH response, proliferation assay of spleen lymphocytes, and clearance ability for C.t challenge from genital trac.Results:1 Inoculating rMOMP vaccine and DNA vaccine could induce C.t specific cell mediated immunity (CMI) and humoral immunity to some extend. It could protect mice from C.t vagina challenge.2. Freund's adjutants could dramatically enhance the CMI induced by C.t rMOMP vaccine.3. Chlamydia trachomatis MOMP DNA connects with rMOMP vaccine can enhance protecting immune responses.4. The mice didn't induce Chlamydia-specific humoral and cellular immune responses by intravaginal.Conclusion:1. rMOMP vaccine and DNA vaccine of C.t E serotype exhibited some immunoprotection effect in mice, but the immune responses induced in mice vaccinated with rMOMP are better. The mice didn't induce Chlamydia-specific humoral and cellular immune responses by intravaginal.