Transhepatic Artery Embolization Combined With En Du For The Treatment Of Hepatoma

Objective:To establish New Zealand rabbit hepatoma models with VX2 cell line and analyze inhibitory effect and safety on rabbit VX2 hepatoma by Transhepatic arterial chemoembolization(THAE)combined with re-endostatin via hepatic artery.Materials and Methods:VX2 carcinoma cells were implanted into the right liver lobe in 20 male white rabbits with digital subtractive angiography (DSA)., which were randomly divided into 4 groups. Group A was treated with Physlogical saline via hepatic artery. GroupB was treated with THAE only. GroupC was treated with endostain via peritoneal injection. Group D was treated with THAE combined with ren-endostatin via hepatic artery. The VX2 hepatomas were observed after implantation at the twist weeks with multi-slice spiral CT scan including plain and enhanced scan was performed. The expression of VEGF and microvessel density (MVD) value of the tumors were measured on the 14th day after treatment respectively. The data was statistically analyzed by SPSS 11.0 statiastial bag.Results:1 After the treatmeat, There were no animals died in Group A,C. One animal was died in B.One animal's insion disunioned in Group D. Most of them were in depress-ion and drowisiness and moved little.2.The tumors demonstrated as hypodensity or isodensity on plain CT, enhanced or ring-enhanced on arterial phase, hypodensity on portal phase.3 The expression of VEGF value of the tumors of Group D was more lower than other treatment groups.The mean OD was (0.0210±0.0043),and the differenses between them were statficly significant (P<0.05), The expression of VEGF of GroupB was the highest. The mean OD was (0.0714±0.0030), and the differenses between GroupA and Group B was statficly significant (P<0.05), The mean OD of VEGF of Group D was (0.0302±0.0022) and was lower than GroupB and A.4 The expression of microvessel density (MVD) value of the tumors of Group D was more lower than other treatment groups. The mean OD was (0.0023±0.0002) and the differenses between them were statficly significant (P<0.05), The expression of MVD of GroupB was (0.0078±0.0004) and was the highest.The differenses between GroupA and Group B was statficly significant (P<0.05), The expression of MVD was (0.0041±0.0004) and was lower than GroupB and A. (P<0.05),Conclusion:The short-term effect on rabbit VX2 hepatoma of THAE combined with re-endostatinvia hepatic artery infusion is superior to that of other groups in suppressing tumorangiogenesis and re-endostatin causes no more serious kidney and liver dysfunction.