Expression Of ERCC1,BRCA1 And Ki67 And Their Biological Significance In Lung Cancer Tissue Microarry

ObjectiveTo detect the expressions of ERCC1, BRCA1, Ki67 in lung cancer progression tissue microarray by immunohistochemistry and discuss their roles in lung cancer genesis and progress. With these results, we wanted to put our discoveries into the clinicopathological diagnosis, prognosis evaluation and gene therapy.Material and MethodWe used Envision immunohistochemistry method to detect the expressions of ERCC1, BRCA1 and Ki67 in tissue microarray with 270 cores, and analyzed the relationship between their expressions and clinicopathological parameter.Results1. The positive rate of ERCC1 in primary lung cancer and precancerous lesion were higher than that of normal lung tissue(P<0.05). The positive rate of ERCC1 was higher in the group of peripheral than central type(P<0.05), higher in the group of high-middle differdntiation than low-un differentiation, higher in the group ofâ… +â…¡stage thanâ…¢+â…£stage (P<0.05).2. The positive rate of BRCA1 in primary lung cancer and precancerous lesion were higher than that of normal lung tissue(P<0.05). The positive rate of BRCA1 was significantly lower in SCLC than NSCLC. The positive rate of BRCA1 was higher in the group of high-middle differdntiation than low-un differentiation (P<0.05), it was higher in the group ofâ… +â…¡stage thanâ…¢+â…£stage (P<0.05).3. The positive rate of Ki67 in primary lung cancer was higher than precancerous lesion and normal lung tissue(P<0.05). The expression of Ki67 was higher in the group of low-un differentiation than high-middle differentiation (P<0.05). The positive rate of Ki67 was higher in the group with lymph node metastasis than that without lymph node metastasis (P<0.05).4. There was significantly positive correlation between ERCC1 and BRCA1 (P<0.05). There was significantly negative correlation between BRCA1 and Ki67 (P<0.05). Conclusions1. ERCC1 had an impotant effect on tumors. The positive rate of ERCC1 was higher in primary lung cancer and precancerous lesion than that in normal lung tissue, it indicates that in the early stages of lung cancer genetic damage already exists. The expression of ERCC1 had correlation between eye type, differentiation and clinical stage, so it can be a reference in prognosis evaluation. It indicates that lower expression of ERCC1 have a higher invasiveness and poor prognosis.2. The positive rate of BRCA1 was higher in primary lung cancer and precancerous lesion than normal lung tissue. It indicates that the expression of BRCA1 was related to the occurrence and development of lung cancer. The positive rate of BRCA1 was significantly lower in SCLC than that in NSCLC, which suggested it can be a marker to distinguish SCLC and NSCLC. The expression of BRCA1 had correlation between differentiation and clinical stage, it indicates that BRCA1 may have the effect that inhibit the proliferation and differentiation of the tumor cells.3. The positive rate of Ki67 was higher in primary lung cancer than that in precancerous lesion and normal lung tissue. The positive rate of Ki67 was significantly related to the degree of differentiation and lymph node metastasis, which suggested it can promote the malignant transformation and progression, so it has a reference in prognosis evaluation.4. There was significantly positive correlation between ERCC1 and BRCA1. There was significantly negative correlation between BRCA1 and Ki67. The study on the relationship of ERCC1, BRCA1, Ki67 can reveal the mechanism related to lung cancer genesis and progress and provide theoretical evidence for gene therapy.5. Tissue microarray has the advantage of high throughput, high efficiency, saving reagent and time. It can reduce the experimental error and have prominent advantage in large scale research.