The Extraction And Isolation Of Polypeptide With Analgesis Activity From Scolopendra Subspinipes Mutilans

In traditional Chinese medicine clinical use in the centipede of the boiled liquid with a clear Qufeng analgesic, attacking the role of drug Sanjie, this issue aimed at finding a centipede in the best analgesic peptide extraction process, analgesic peptide purification and separation technology the size of its analgesic activity, in order to develop safe and effective analgesic peptide product formulations to provide a basis for the high-tech medicine centipede analgesic peptide products into the market, large-scale production basis for the majority of patients with a better centipede preparations safer choice, the modernization of traditional Chinese medicine for animal research to draw on the example.Refluxed with ethanol and water-soluble alcohol precipitation technique to extract the centipede polypeptide composition of protein. Using three factors and three levels of the orthogonal design method to extract rate and nitrogen content as an indicator comprehensive consideration to the centipede protein derived peptides to bring the best process route.Use of alginate adsorption, salt precipitation, the method of the crude protein extract enriched peptides, and then the use of Sephadex G25\G50 column and RP-HPLC separation techniques such as active on thetarget peptides were separated by to analgesic activity intensity as apharmacodynamic indicator of analgesic activity purified from the centipede with a single polypeptide composition, molecular weight determination of the size of its structural characteristics.Through mice by intraperitoneal injection of fresh acetic acid so thatit made the pain of the acetic acid writhing model, water and aspirin as a positive and negative control, research and determination of centipede peptide analgesic activity and the activity of the size.This topic was first introduced to the centipede centipede that has analgesic activity extract isolated polypeptide composition, find the best centipede peptide extraction process line, to find centipedes polypeptide purification techniques, to be centipedes peptide monomers. Centipede analgesic peptide monomers of the structural determination of molecular size, understanding its mechanism. Determination of acetic acid writhing through the centipede extracts and the peptide monomer centipede the size of the analgesic activity. Analgesic agents for the Centipede basis for the study. The final purified from a subject with a molecular weight of about 8KD analgesic activity of the weak acid peptide monomers centipede. Centipede for our traditional Chinese medicine, the subject of applications for the modernization of Chinese medicine can learn from example. Centipede peptides as non-addictive analgesic effect, the use of analgesics addiction to speed up the analgesic of the world's abuse and the stability of society cause great security risk to society caused by a serious threat to peace, This issue mostly for non-analgesic drug addiction and market research provide theoretical foundation and technical guidance.