| Objectives To research the changes of the tumor necrosis factor-α, IL-1β, interleukin-6, nuclear factor-κB, ICAM-1 of the mucosa after the application of glycyrrhizin in rats with severe acute pancreatitis, To study the possible effect mechanism of glycyrrhizin on severe acute pancreatitisMethods 150 healthy male Sprague-Dawley rats weighing 160-200g, which were randomly divided into sham operationgroup( S group) , severe acute pancreatitis group(P group) and the treatment group with glycyrrhizin of the severe acute pancreatitis (GL group),each group are 50. , the ten detect the survival time of the 72h with each group, P group and GL group are established the SAP model by Puncturing retrograde cholangiopancreatography and pumping 5% sodium taurocholate with intraperitoneal injecting anesthesia through abdominal of the Sodium pento-barbital.Rats in GL group were injected glycyrrhizin after surgery(100mg/kg) ,and at 3h, 6h, 12h, 24h four time points rats were sacrificed in groups using abdominal aortic phlebotomy (n = 10), serum amylas measured by automatic biochemical analyzer ,serum inflammatory cytokines IL-1β, IL-6, TNF-αlevels measured by radioimmunoassay, the activity of NF-κBp65 in pancreatic were detected by immunohistochemistry , at the same time, the scores of pancreatic tissue pathology were detected,the expression of ICAM-1 in ileal mucosa adhesion were detected by immunohistochemistry , at the same time the scores of ileal mucosa pathology were detected and observed the changes of the above indicators after glycyrrhizin pretreatment .All Statistical analysis were performed with SPSS16.0.Main Statistical indicators should be taken normality and homogeneity test,each data were expressed with Mean±standard deviation value,and Several more groups were compared by one-way ANOVA,two groups were analysed by DunnettT3 ,.If the P is less than 0.05 ,It is considered statistical significance. ResultsResults (1) the survival time of GL groupRats compared with the P group, the survival time of GL group were significantly prolonged , P = 0.021 (P<0.05), difference was statistically significant. The content change of the blood serum amylase, TNF-α,IL-1βand IL-6 is: Compared with S group , the P group and GL group were significantly increased at each time point, P group compared with the GL, GL group was lower than SAP group at the same time point,but TNF-α,IL-1βshowed no significant difference in 3 h point, they respectively are :P = 0.280,P = 0.350 (P>0.05), serum amylase,IL-6 showed significantly difference in 3h point , they respectively are :P = 0.023,P = 0.024(P<0.05),but they are significantly different in 6h, 12h, 18h point . In the 6h point they respectively are: P = 0.019,P = 0.012,P = 0.022,P = 0.018 (P<0.05) .In the 6h point they respectively are: P = 0.027,P = 0.024,P = 0.015.,P = 0.013 (P<0.05) In the 6h point they respectively are : P = 0.027.,P = 0.025,P = 0.032,P = 0.015 (P<0.05)(2) the pathological scores of pancreas and intestinal mucosa: P group and GL group were significantly higher than S group at each time point, they are significantly different (P<0.05), Compared with the P group, GL group was lower than P group at the same time point ,It showed no significant difference in 3 h point, they respectively are : P = 0.261,P = 0.378 (P>0.05) but they are significantly different in 6h, 12h, 18h point, the comparison of pancreatic pathology :they respectively are:6h: P = 0.025,12h: P = 0.021,24h: P = 0.019 (P<0.05) .the comparison of intestinal pathology :they respectively are 6h : P = 0.032,12h: P = 0.022,24h: P = 0.017 (P<0.05).(3) the activity change of NF-κB p65 in pancreatic and ICAM-1 in intestinal mucosa: P group and GL group were significantly higher than S group at each time point, they are significantly different (P<0.05), the activity change of NF-κB p65 in pancreatic,GL group was lower than P group at the same time point ,It showed no significant difference in 24h point P = 0.280 (P>0.05), but they are significantly different in 3h,6h,12h point, they respectively are:3h: P = 0.016,6h: P = 0.022,12h: P = 0.025 (P<0.05) .the activity change of ICAM-1 in intestinal mucosa ,GL group was lower than P group at the same time point ,It showed no significant difference in 3h point P = 0.248 (P>0.05), but they are significantly different in 6h,12h,24h point, they respectively are:6h: P = 0.025,12h: P = 0.018,24h: P = 0.022 (P<0.05) .Conclusions 1. Glycyrrhizin may reduce the concentration of TNF-α, IL-1β, IL-6 in the serum of the Severe acute pancreatitis and have inhibit inflammation.2.Glycyrrhizin with glucocorticoid-like effects, may reduce inflammation in severe acute pancreatitis, which may treat SAP to provide a new way in the future... |
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