| Because toxic side effects of chemical medication appear ceaselessly in recent years, voices of searching for savageness medication become highly. Particular curative effects and little side effects of Chinese traditional medicine are paid attention on by the international medicine groups. Schisandra chinensis, one of the Chinese traditional medicine materials have high medical values in traditional medicine. Due to the influence of environment, some Schisandra chinensis with poor qualities can not reach the existing standard, so its speed of going into international trade is restricted. Therefore, it is necessary to develop a method to determine the main component's Schisandra chinensis quickly and accurately. The developed method will also provide a reference for evaluating the quanlity of Chinese traditional medicine materials.An analytical method for determining four lignans of Schisandra chinensis simultaneously by HPLC was reported in the study. The effects of liquid components, liquid amount, detection wavelength, column temperature, flow-rate and injection volume were optimized. The best separation parameters were established:acetonitrile-water was used as mobile phase, and the gradient elution program was chose:during the first 25 min, mobile phase was acetonitrile/water (v/v=50:50); the rato of acetonitrile/water of mobile phase was changed into 70:30 linearly in the following 10 min; the rato of acetonitrile/water of mobile phase was remained as 70:30 during the next 5 min; the rato of acetonitrile/water of mobile phase was changed into 80:20 linearly in the next 4 min; the detector is DAD; the detection wavelengthes of Schisandrin, Deoxyschizandrin, Schisandrin B and Schisantherin A were 216 nm,216 nm,216 nm and 222 nm respectively; the column temperature was 25℃; the flow-rate was 0.8 mL/min; the injection volume was 10.0μL. In the optimized detection condition, the correlation coefficient of four analyts in the range of 0.9994-0.9998 was obtained. The detection limit was in the range of 0.04-0.05μg/mL. The average recovery of samples was in the range of 90.23-95.24%; RSD of the method was in the range of 3.1-4.9%. The method had advantages of good precision, and the simultaneous determination of four elements was realized. A HPLC method was described for the determination of several organic acids of Schisandra chinensis simultaneously. The effects of liquid components, liquid amount, buffer concentration, pH of buffer, detection wavelength, column temperature and flow-rate on the determination were optimized. The best separation parameters were established: methanol-0.01 mol/L NaH2PO3 (pH=2.8) was used as mobile phase; the best gradient elution program was chose:during the first 5 min, the mobile phase is methanol-0.01 mol/L NaH2PO3 (pH=2.8) (v/v=2:98); in the following 11 min mobile phase changes to methanol-0.01 mol/L NaH2PO3 (pH=2.8) (v/v=40:60); the detector was DAD; during the first 4.7 min, the detection wavelength was 210 nm; the detection wavelength was changed to 260 nm in the next 0.6 min, the detection wavelength was returned to 210 nm after 5.3 min; the column temperature was 25℃; the flow-rate was 0.8 mL/min; the injection volume was 10.0μL. In the optimized detection condition, the correlation coefficient of seven analyts was in the range of 0.9990-0.9999. The detection limit was in the range of 0.01-2.5μg/mL. The average recoveries of samples was in the range of 90.29-102.52%; RSD of the method was in the range of 1.0-4.6%. This method was successfully used to determine several organic acids rapidly.The experimental results of the research suggested that two analytical methods for the determination of main components of Schisandra chinensis were sensitive and accurate.
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