| Objective:Studies of brain-derived neurotrophic factor (BDNF) on neonatal rat hypoxic-ischemic brain damage (hypoxie-ischemic brain damage, HIBD) and its possible mechanism, provide the basis for the treatment of hypoxic-ischemic brain injury and its related diseases。Methods :Two hundred and fifty-six 7-day-old Wistar rats were randomly divided into four groups: normal control group(n=64,A);separation of the left common carotid artery only;HIBD model group(n=64,B);separate and ligate left common carotid artery ; model pre-treated group(n=64,C);separate and ligate left common carotid artery after inject BDNF;model after the administration group (n=64, D),inject BDNF after ligate left common carotid artery ,every group respectively divided into 1d,3d,5d,7d four time points (n=8)。The expression of Trk-B and P75 in Hippocampus was examined with immunohistochemical staining and real-time PCR。 Results:(1)Compared with A and B, the C,and D groups at four time points showed Trk-B levels were significantly elevated, p75 expression levels decreased, the most significant change in C group,the difference was statistically significant(P<0.05)。D group and A, B there was a marked change in the two groups, the difference was statistically significant (P<0.05), (2)The performance of real-time fluorescence quantitative PCR for the C, D compared with groups A, B ,Trk-B gene expression levels increased,p75 gene expression levels decreased, the most obvious changes in group C, and the same trend of changes in immunohistochemistry。Conclusion:(1)Injection of BDNF in advance plays an important role toimprove the prognosis of HIBD; after injection may improve the prognosis of HIBD;(2) BDNF in advance to give the prognosis of HIBD more effective than after injection; The possible mechanism is that BDNF-induced Trk-B can be produced in advance and suppress the expression of p75, thus play a role by increasing the expression of Trk-B levels and reduce the expression level of p75。... |
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