Detection Of Chromsomal Abnormalities In Patients With Multiple Myeloma By Fluorescence In Situ Hybridization

Objective:To investigate the incidence of common chromosomal abnormalities of the patients with multiple myeloma by fluorescence in situ hybridization.Methods:By fluorescence in situ hybridization, five specific probes including 1q21,D13S319,RB-1,IgH,p53 were used to study the chromosomal abnomalities of 18 patients with multiple myeloma.Results:Among the 18 cases of multiple myeloma patients, there were 13 cases of chromosome abnormalities emerged, the overall detection rate of chromosomal abnormalities was 72%(13/18). And the frequencies of abnormalities in 1q21, the deletion of chromosome 13 (including D13S319 and RB-1 two probes), translocation involving the 14q32 region, and deletion of chromosome 17 were 6%(1/18),33%(6/18),33%(6/18), 6%(1/18), respectively. One case there were two kinds of chromosomal abnormalities while IGH and D13S319. Analysis showed that, there was no correlation between the incidence of chromosomal abnormalities and that the sex,age,D-S stage,ISS stage,hemoglobin,platelets,plasma cells,β2-MG,albumin,creatinine,lactate dehydrogenase,C-reactive protein of the patients with multiple myeloma.Conclusion:1. Fluoresecence in situ hybridization is a more rapid, sensitive and accurate technique in the analysis of multiple myeloma in patients with abnormal chromosome. The the deletion of chromosome 13 and the translocation involving the chromosome 14 was more common.2. The chromosome abnormality was unrelated to the sex,age,D-S stage,ISS stage,hemoglobin,platelets,plasma cells,β2-MG,albumin,creatinine,lactate dehydrogenase,C-reactive protein of the patients with multiple myeloma...