Studies On The Polar Chemical Constituents Of Pachysandra Terminalis And Their Biological Activities

Pachysandra terminalis Sieb.Zucc is the whole plant of Pachysandra terminalis (Buxaceae).It widely distributes in the southwest and northwest of the People's Republic of China. There are three genus in the Family of Buxaceae genus, they are Sarcococca, Pachysandra, Buxus. As a Chinese folk medicine, Pachysandra terminalis Sieb.Zucc has been usually used to dispel wind, activating blood, promoting menstruation.In order to search the bioactive constituents from natural source, we began to study the chemical consitituents from Pachysandra terminalis Sieb.Zucc. By repeated column chromatography, including Silica gel, Toyopearl HW-40 and macroporous adsorption resin, and preparative HPLC,16 compounds were isolated from 95% EtOH extract of Pachysandra terminalis Sieb.Zucc. By using IR, MS, 1H-NMR,13C-NMR,1H-1H COSY, HMBC, HSQC, NOESY spectra and chemical methods, nine new compounds,4-methyl-3-methylenepentane-1,2,5-triol (1), 4-methyl-3-methylenepentane-1,2,5-triol (2),3-isopropenyl-butane-1,2, 4-triol (3),4-methyl-3-methylenepentane-1,2,5-triol-1-O-β-D-glucopyra noside (4),3-methylenehexane-1,2,5-triol-1-O-β-D-glucopyranos ide (5), (6R, 7E,9R)-9-methylamino-4,7-magastigmedien-3-one, (6R, 7E,9S)-9-methylam ino-4,7-magastigmedien-3-one (7), Pachysandrol A (8), epipinoresinol-3-O-β-D-glucopyranoside(9), along with seven known compounds were identified. Compound 1-16 were isolated from Pachysandra terminalis Sieb.Zucc for the first time. Polyhydroxy alkanes were isolated from this plant for the first time and were the characteristics constituents of Pachysandra terminalis Sieb.Zucc.The immunosuppressive activities of different extracts and isolated compounds from Pachysandra terminalis Sieb.Zucc were also investigated. The n-BuOH extract revealed significant immunosuppressive activities. Their antioxidant activity and immunosuppressive activity had been investigated. The results showed that compound 10,13～16 had significant antioxidant activity, compound 1,2,11～14 had lower immunosuppressive activity.