Studies Of Chemical Constituents And Antioxidant Activity Of Different Fractions From Uygur Medicine Ziziphora Clinopodioides Lam

Objective: This study was designed to examine the chemical composition and antioxidant activity of Uygur medicine Ziziphora clinopodioides Lam., to further expand the pharmacology effect of Ziziphora clinopodioides Lam. and to establish firm fundament of reasonable developing and utilizing to Ziziphora clinopodioides Lam. in Xingjiang. Method: Through colorimetric method to determine contents of multi-polyphonic,total falconoid in alcohol extracts of Ziziphora clinopodioides Lam. with different heteropolarity extraction parts (petroleum ether,chloroform,acetic ether,n-butanol and alcohol); applied to the method of high performance liquid chromatogram (HPLC) to determine the contents of oleanolic acid,ursolic acid in Ziziphora clinopodioides Lam. from eight different places of production in Xinjiang. Use GC/MS technique to analyze the chemical composition of essential oil from Ziziphora clinopodioides Lam., meanwhile conduct the investigation and research with the stability of essential oil. Utilize four types of antioxidant activity models (clear DPPH free radical,hydroxide radical,anti-superoxide anion free radical and SOD active effect) do antioxidant activity screening to alcohol extracts of Ziziphora clinopodioides Lam. with different heteropolarity extraction parts. Results: Multi-polyphenol and total flavonoids in alcohol extracts of Ziziphora clinopodioides Lam. with different heteropolarity extraction parts main centered in acetic ether extraction part, which account 19.27 % and 65.6 % respectively; the contents of oleanolic acid,ursolic acid in Ziziphora clinopodioides Lam. from eight different places of production in Xinjiang existed obvious difference; illumination,temperature,heavy metal ion and acid environment have effects on the stability of essential oil, identified 37 species of chemical compound from the essential oil; the antioxidant experiment proved the acetic ether part of Ziziphora clinopodioides Lam. has good antioxidant activity. Conclusion: The study determined contents of multi-polyphenol,total flavonoids in alcohol extracts of Ziziphora clinopodioides Lam. with different heteropolarity extraction parts, and determined contents of oleanolic acid,ursolic acid in Ziziphora clinopodioides Lam. from eight different places of production in Xinjiang. It offered the theory evidences for establishing scientific quality standard of Ziziphora clinopodioides Lam.; meanwhile, firstly conducted antioxidant activity screening to alcohol extracts of Ziziphora clinopodioides Lam. with different heteropolarity extraction parts, searched the active part which has good antioxidant activity.