Expression Of ILK And E-Cadherin In Bladder Transitional Cell Carcinoma And Its Significance

Objective:To investigate the transcription and expression of ILK mRNA,protein and E-cad in bladder transitional cell carcinoma (BTCC) and explore their significance in carcinogenesis and progression of BTCC.Methods:1.The expression of ILK mRNA in 56 cases of BTCC and 30 cases of adjacent normal bladder tissues were examined by reverse transcriptase polymerase chain reaction (RT-PCR).2.The expression of ILK protein and E-cad in 56 cases of BTCC and 30 cases of adjacent normal bladder tissues were examined by Immunohistochemistry.Results:1.The positive rates of ILK mRNA in BTCC and adjacent normal bladder tissues were 58.9%(33/56) and 13.3%(4/30), respectively. The expression level of ILK mRNA in BTCC was significantly higher than that in adjacent normal bladder tissues (P<0.05). The expression rate of ILK mRNA in BTCC was positively correlated with tumor grade and lymph node metastasis (P<0.05), and with the increase of clinical stage, ILK mRNA expression gradually increased, but there is not significant (P> 0.05).2.The positive rate of ILK protein in BTCC and adjacent normal bladder tissues were 53.6%(30/56) and 10%(3/30), respectively. The expression level of ILK protein in BTCC was much higher than in adjacent normal bladder tissues (P<0.05), which was related positively with pathological grade and lymph node metastasis (P<0.05), but was not correlated with the clinical stage (P>0.05).3. The positive rate of E-cadherin protein in BTCC and adjacent normal bladder tissues were 51.8%(29/56) and 96.7%(29/30), respectively. The expression of E-cadherin protein in BTCC, was closely correlated with pathological grade (P<0.05), clinical stage (P<0.05) and lymph node metastasis (P<0.05).4. Statistics results showed that ILK protein was negatively correlated with E-cadherin protein (rs=-0.48, P<0.05).Conclusions:1. The high level expression of ILK mRNA and protein are related with pathological grade and lymph node metastasis (P<0.05), but no correlation with the clinical stage (P> 0.05), it is suggested that ILK may be involved in BTCC carcinogenesis and metastasis, and is closely related to their degree of malignancy, but is not related to tumor infiltration. 2. Low expression of E-cadherin protein in bladder transitional cell carcinoma, are related with pathological grade, clinical stage and lymph node metastasis, it is suggested that E-cadherin may be involved in BTCC carcinogenesis, development, differentiation and metastasis.3. High expression of ILK protein and loss of E-cad expression are significantly related to tumor progression in BTCC, they might have synergistic effect in the occurrence and metastasis of BTCC, it is suggested that they may become potential targets for determining the degree of differentiation and metastasis.