The Effect Of Chronic Intermittent Hypoxia On Blood Glucose And Pancreatic Islets B-cell Ultrastructure In Rats

Objective:To set up a chronic intermittent hypoxic animal model, as an experimental platform for further study of the pathogenesis of OSAHS. To detect the blood glucose of rats in each group,the fasting blood insulin and C-peptid,and then get the insulin resistance index,and observe the pancreatic islet B-cells under transmission electron microscope of rats in each group, to provide a theoretical basis for the reconstruction, the intervention earlier period and the treatment of patients with OSAHS.Methods:Experiments were performed on 24 three-month-old male Sprague-Dawley rats, they were randomly assigned to three experimental groups (n=8/group): unhandled control group (UC), chronic intermittent hypoxia group (CIH), and Removal of hypoxia group (RH). Rats in UC group were raised normally, those in CIH group suffered 8H intermittent hypoxia everyday, the duration of experiment was 4 weeks, and those in RH group was the same as those in CIH group in previous 4 weeks and were raised normally in later 4 weeks. All the rats were killed after experiment. The FBG,the FINS and the C-PEPTIDE of rats were measured, and the IRI can be got by calculation, in the same time we observe the pancreatic islet B-cells under transmission electron microscope.Results:â‘ The level of the rats'FBG in the CIH group increased significantly than those in the UC group and RH group (P<0.01), but it is not true between UC group and RH group (P>0.05);â‘¡The level of the rats'IRI in the CIH group increased significantly than those in the UC group and RH group (P<0.01), but it is not true between UC group and RH group (P>0.05);â‘¢The ultramicrostructures of rats'the pancreatic islet B-cells had slight changes of hypoxia in the CIH group.Including a lot of the myeloid body ,the endoplasmic reticulum dilate ,the bioblast slight engorge and cavitation ,the secretory granule cavitation increased but the quantity had not been changed obviously.but they were normal in UC group and RH group.The appearance and quantity of Golgi complex had not been changed obviously in all rats.Conclusions: Chronic intermittent hypoxia can lead to rats'the pancreatic islet B-cells damage and its function disturbance.there was the hyperinsulinemia, the insulin resistance and the fasting blood glucose increased.but restore hypoxia can make it normal.