Abnormal DNA Repair Capacity And Apoptosis Of Ovarian Cancer And Cisplatin Resistance In Relationship

Objective (1)Observation of cisplatin led to SKOV3 human ovarian cancer cell apoptosis after drug resistance。To investigate the roles of hMLH1 and hMSH2 gene in cisplatin resistance in human ovarian cancer. (2)To investigate the roles of bax,bcl-2 and caspase9 gene in cisplatin resistance in human ovarian cancer. To provide the apoptosis way for laboratory further study.Methods (1)The chemosensitivity to cisplatin was measured by MTT assay. The apoptosis induced by DDP was tested by Hoechst fluorescence analysis. The gene expression of hMLH1 and hMLH2 at transcription and translation levels in SKOV3 and SKOV3/DDP were measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, respectively. (2)The gene expression of bax,bcl-2 and caspase9 at transcription and translation levels in SKOV3 and SKOV3/DDP were measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, respectively.Results (1)The apopototic rate of SKOV3/DDP was markedly reduced compared to that of SKOV3 after cisplatin treatment. Both mRNA and protein levles of hMSH2 in SKOV3 were significantly higher than those observed in SKOV3/DDP. (2)Both mRNA and protein levles of bax and caspase9 in SKOV3 were significantly higher than those observed in SKOV3/DDP. mRNA and protein levles of bcl-2 in SKOV3 were significantly lower than those observed in SKOV3/DDPConclusion The inhibition of hMLH1 and hMSH2 gene expression may contribute to the occurrence of cisplatin resistance in human ovarian cancer.Also The inhibition of bax,bcl-2 and caspase9 gene expression may contribute to the occurrence of cisplatin resistance in human ovarian cancer...