Effect On Arsenic Metabolites And Biochemical Indexes In Rat Organs Of Different Valence State Of Inorganic Arsenic Exposure

Objective: To observe the different valence, different doses of arsenic (NaAsO2 or Na2HAsO4) exposure in rats, the liver, brain and kidney morphological differences; by measuring the different valence, different doses of arsenic in different tissues and organs after exposure to arsenic metabolites, The mechanism of arsenic poisoning for the more scientific and theoretical basis; arsenic metabolism through the analysis of biochemical indexes reveal the different forms of arsenic and related biochemical indexes of relevance; also examine some indexes of oxidative damage, in order to further explore the arsenic poisoning provide the basis for the pathogenic mechanism. Methods: 70 Wistar rats were randomly divided into control group (C), iAs³⁺ low, middle, high dose groups, iAs⁵⁺ low, middle, high dose groups, total of 7 groups. Exposed by the free water method. Continuous exposed for 90 d. Measure the contents of DMA, and MMA in the liver, brain and kidney homogenate by HPLC-HGAFS; Measure the vitality of GSH and GST of the liver, brain and kidney by ultraviolet spectrophotometry; Measured GSH-Px activity, GSSG content, GAPDH content by double antibody sandwich method; Measured methyltransferase activity by enzyme linked immunosorbent assay. Results: 1. The experimental groups had varying degrees of weight gain slowed down in the arsenic accumulation in tissues increased amount of the performance of sub-chronic arsenic poisoning; 2. Different valence of different doses of arsenic are likely to undermine the liver and kidney tissue of normal structure; 3. Organs of rats in each experimental groups GSH-Px activity lower than the control group (P <0.05), and with the increase of Arsenic dose, showed a downward trend; large experimental groups GST activity of mouse organs than normal control group (P <0.05), and with the increasing doses of Arsenic, iAs³⁺ groups showed a downward trend, iAs⁵⁺ group between high and low dose was no significant difference (P> 0.05); BH GSSG activity in rat organs were higher than the control group (P <0.05), the other organs in the experimental groups GSSG activity lower than the control group (P <0.05); the GSH organs of experimental groups different levels with the control group (P <0.05), AL, BL and BM groups showed higher than normal control group, the other experimental groups showed lower than normal group; organs of rats in each experimental group were higher than normal GAPDH the control group (P <0.05), iAs⁵⁺ groups were higher than iAs³⁺ groups (P <0.05); organs of rats in each experimental group methyltransferase activity levels higher than the control group (P <0.05), iAs⁵⁺ groups were higher than iAs³⁺ group (P <0.05), with the group no significant difference between male and female (P > 0.05); 4. The correlation analysis of liver and kidney of methyltransferase correlated with the DMA content (P <0.01); liver , brain, and kidney of methyltransferase activity was positively with the MMA correlate (P <0.01). Conclusion: iAs⁵⁺ and iAs³⁺ were exposed to liver and kidney after the obvious injury. iAs⁵⁺ and iAs³⁺ can decrease the activity of antioxidant enzymes in the body, oxidative damage and antioxidant system in dynamic equilibrium, resulting in body lipid peroxidation. Organs of GAPDH activity and effectiveness of pentavalent arsenic reduction are closely related. Valence effects of inorganic arsenic methylation of arsenic.