Changes Of EPO - EPOR Pathway In Chronic Stress Rats And The Effect Of Sini Powder On Its Regulation

ObjtctiveThe study observe chronic stress depression model rats as the research object, research EPO-EPOR pathways in the change of serum, liver, heart, kidney, and the adjustment of Sini san.Preliminary discuss the role of EPO-EPOR pathways in depression, and Sini san’s mechanism of treatment.Method(1) 50 healthy male SD rats, weight (130±10) g, adaptive rats feeding 1 week before experiment, free to eat, drink. After the Open-field Test (OFT),according to the weight randomly divided the rats into control group (n=10);Model group (n=10), give different Chronic Unpredictable Mild Stress(CUMS) stimulation;Fluoxetine treatment group (n=10), given CUMS stimulation, daily irrigation fluoxetine hydrochloride solution, daily dose of 3 mg/(kg* d); Sini san Treatment group (n=10), give CUMS stimulation, filling and Sini san decoction,daily dose of 4.2 mg/(kg*d); EPO Treatment group (n=10), give CUMS stimulation, daily intraperitoneal inject recombinant human erythropoietin (rhEPO),5000 UI/kg. Modeling peroid sustaining for 42 days. Body weight, open field test and sucrose preference test were measured every two weeks, respectively.(2) Using enzyme-linked immunosorbent assay (ELISA) to detect the content in serum of tumor necrosis factor-α(TNF-α)and the EPO content in serum, liver, heart and kidney.(3) Using Western Blot and immunohistochemistry assay liver, heart, kidney’s EPOR expression levels of each group.Results(1) Model group significantly lower than the control group(P<0.05) of body weight, no significant changes after the Sini san treatment(P>0.05).Open-field test results show that the model group was significantly less than the control group in total score(P<0.05),and increased significantly after Sini san treatment(P<0.05). Sucrose preference test showed that the model group significantly reduced in the percentage of sucrose preference(P<0.05) than the control group,and no significant change after Sini san treatment(P>0.05).(2) ELISA results showed that the TNF-a content in serum of model group significantly increased(P<0.05),and after EPO treatment significantly reduced(P<0.05).(3) ELISA results showed that compared with the control group, the model group is significantly increased the EPO content in serum, liver,and heart(P<0.05).And Sini san can significantly reduce the EPO content in serum and heart(P<0.05).(4) Western Blot, immunohistochemistry results showed that compared with the control group, the EPOR expression in model group is significantly increased in the kidney and liver(P<0.05).The expression level of EPOR in kidney decreased significantly after Sini san treatment(P<0.05); in the heart, model group rats’ EPOR expression levels were significantly reduced(P<0.05), significantly increased after Sini san treatment(P<0.05).Conclusions(1) In rat model of depression, there are changes of EPO-EPOR pathway in multiple organs and Sini san can adjust these changes.(2) The EPO-EPOR pathway is likely to be one of the modern biology foundation of TCM liver’s’storing blood and controlling converyance and dispersion’ function.