Study On Key Technology For The Stable Yields Of Coronatine Of Pseudomonas Syringae Pv. Mori

The coronatine is a new-type and high-efficient biogenic plant growth regulatingsubstances. Plants would get higher stress resistance and disease resistance with sprayingcoronatine in a low concentration, which has broad application prospects. It’s an effectiveway to ferment coronatine industrially using Pseudomonas syringae. The precondition is thestrain that produces stable and high yield coronatine. In this paper, quantitative culturetechniques were used to research density distribution of Pseudomonas syringae pv.moriunder different micro-ecological environment. Repetitive-element PCR was used to classifythe Pseudomonas syringae pv.mori from different ecological environment. Strainsproducing coronatine were mutanted by the Atmospheric room-temperature plasmatechnology. Results suggest that the wild strains producing coronatine could be effectivelyscreened from mulberry leaf tissues which are sensitive to the Pseudomonas syringaepv.mori. Mutants produce coronatine largeduty and stably were available using theAtmospheric room-temperature plasma technology. Research results lay the foundation forlarge-scale industrial fermentation of coronatine.1) The wild strains producing coronatine could be effectively screened from mulberryleaf tissue.Pseudomonas syringae pv.mori wild strains from different regions were screened usethe KBC selective media technology, density distribution of Pseudomonas syringae pv. moriunder different micro-ecological environment were researched. The results showed thatthere were significant differences in the number of Pseudomonas syringae of mulberry leaftissue and soil. It was found that mulberry leaves showed greater population density ofPseudomonas syringae pv.mori. This is correlation with infection way that Pseudomonassyringae secrete coronation to regulate plant stomata in the occurred process of mulberrybacterial blight. The results found a factual basis for the screening of high-yieldPseudomonas syringae wild-type strains of coronatine from mulberry leaves tissue in theoccurred process of mulberry bacterial blight. 2) The mulberry field abundant water system is the most potential target source to selectthe wild strain producing high-yield coronatine.The REP universal primers were used to establish the repetitive sequence PCRtechnology. Researches on clustering analysis and classification of the coronatine producingwild strains included in the culture collection that isolated from Jiangsu and Zhejiang areawere worked. The results show that29strains were divided into five groups in the similaritycoefficient of0.65. The cluster analysis showed the mulberry organization from the Chun’ancounty that can selected more Pseudomonas syringae pv. mori due to its high moistureconditions. Thus speculated that some relevance between the regional water conditions anddiversity of wild strain. That provides a new train of thought for the screening of the wildstrain producing coronatine3) Mutant strains that producing coronatine largeduty and stably can be obtained by theAtmospheric room temperature plasma technology.The yield of coronatine of the original wild strain was analyzed. It showed that theoriginal strains can produce coronatine at high temperature (32℃). But the yield ofcoronatine was not stable and the property of coronatine declined obviously. The originalstrain was mutated with the Atmospheric room temperature plasma technology and culturedat general medium. Results show that the yield of coronatine of the original strain hasincreased by5%and it is stable from1to10generations that the amplitude variations arewithin2.24%. Keeping a stable and high-yeild property of coronatine provides foundationfor large-scale industrial production. Therefore, the Atmospheric room temperature plasmatechnology has improved the stability of the strain yield effectively on the premise ofkeeping character of high yield. It provides a novel and effective technology for the efficientscreening of strains for industrial production of coronatine.The paper shows that it is screened the Pseudomonas syringae pv. mori from themicroecosystem of ecological mulberry field firstly. The Atmospheric room-temperatureplasma technology was used to mutant the Pseudomonas syringae pv. mori. The mechanismof improvement of coronatine was discussed. Key technologies of producing coronatinelargeduty and stably were researched. It has important and practical significance for industrialization of high-yield coronatine and monitoring and prevention the mulberrybacterial blight disease.