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Study On Bioactivity Screening Of Endophytic Fungi From Pogostemon Cablin And Metabolites Of Myrothecium Roridum

Posted on:2015-06-18Degree:MasterType:Thesis
Country:ChinaCandidate:W Z LiuFull Text:PDF
GTID:2180330452953765Subject:Pharmacy
Abstract/Summary:PDF Full Text Request
Endophytic fungi, lives in different organization of healthy plants, which doesnot lead obvious symptoms disease to plants. Most endophytic fungi and the hostbenefits from each other, Moreover, endophytic fungi are able to produce secondarymetabolite which sames or closes to the host chemical composition. According to thereport, endophytic fungi secondary metabolites behavior virous biological activity,such as anti-tumor, antibacterial, antioxidant, anti-virus and so on. Therefore,endophytic fungi secondary metabolite becomes the material origin of new drugsresearch. This paper gets38endophytic strains from the Pogostemon cablin whichpacked in Guangdong Pharmaceutical University, University City Campus. Throughscreening the compound abundance of crude extracts, antimicrobial activity test andcytotoxic activity, Myrothecium roridum behaviors inhibiting the activity of HepG2、SF-268、NCI-H460evidently, the crude extracts inhibited the growth rate94.3%,88.7%and83.8%in the concentration100g/mL, respectively. Through the bulkfermentation and separation of metabolites, some compounds exhibiting significantcytotoxic activity are expected.Objective: To screen out the high activity endophyte strains from Pogostemoncablin and research on active substances in endophytic fungi Myrothecium roridum.Method: Principal expedition on cytotoxic activity and antimicrobial activity of thecrude eatract. Using Positive phase silica gel column chromatography, SephadexLH-20, C18reverse phase, HPLC, preparative thin layer chromatography,recrystallization and other methods separating and purifying compounds. Through the determination of the physical and chemical constants and spectroscopic data analysisand other means (1H-NMR,13C-NMR, ESI-MS, EI-MS DEPT135, HSQC,1H1H-COSY, and HMBC) to identify the structure; taking SF-268、MCF-7、NCI-H460、HePG-2as the selected cell lines, by using the SRB dyeing to conduct in vitrocytotoxin activeness research to the compound. Taking Candida albicans as the testedstrain, by the filter paper dispersion method to study the antimicrobial activity ofcompound, in vitro. Result:13high active strains were screened out from38endophytic strains.20compounds were separated from this Myrothecium roridum,14of them have identified by the spectral data analysis and contrast samples, they are:Hydroxytyrosol (MR1),2-hydroxy-6-(2-hydroxypropyl)benzoic acid (MR2),1,3-di-O-[2,2di-(p-phenylene) isopropylidene] glycerol diphenol (MR3), epiroridin E(MR4), mytoxin B (MR5),4-hydroxybenzaldehyde (MR6), nepethalate A (MR7),16-Carbonylroridin E (MR8), sterols (MR9), uridine (MR10),1-methylpyrrolidin-2-one (MR12), chrysophanol (MR13),5-hydroxymethyl-furfural(MR14), Ergosterol3-O-b-D–glucopyranoside (MR15). Conclusion:34.2%of theendophytic strains could produce active crude axtract. Compounds MR1、MR2、MR3、MR6、MR7、MR8、MR12-MR15are the first time isolated from the strain secondarymetabolites. Compounds MR3、MR4、MR5、MR8、MR10show significant activitywith SF-268、MCF-7、NCI-H460、HePG-2. MR9show significant inhibition. MR4show certain antibacterial activity with Candida albicans, and the new compoundMR8is lower than known compound MR4slightly in this biological activity.
Keywords/Search Tags:Pogostemon cablin, Endophytic fungi, activity screening, Myrotheciumroridum, Secondary metabolites
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