Difference Of MRNA Secondary Structure And MFE Among Different Spieces

RNA is an important biological organism which is indispensable in various life activities, not only including the includes transfer of genetic information, but also other important biological functions such as the process of RNA splicing and modifying, it also can play as a catalyst in the process of RNA splicing, and can play a regulatory role in the process of translation, which involves degradation of RNA and localization in cell. There are many different kinds of RNA in cells, including tRNA(transport RNA),rRNA(ribosomal RNA), and mRNA(messenger RNA), and other non-coding RNA. The first tree types of RNA involved in protein synthesis in the cell. In addition, RNA has important biological functions. Like in splicing and modification of RNA, and gene silencing process.RNA single molecules tend to fold into double strands in all kinds of cells. When RNA folds, it also constructs specific structures which plays important roles in various life processes. These includes protein folding and transporting, the start of translation and extension. RNA’s structure can also affect the translation rate and the stability of mRNA. mRNA involves in a variety of cell metabolism, like a specific protein’s formation and binding to other biological molecules. mRNA also has the task of controlling life process. Besides the structure, another form for mRNA to play a part in life process is with the stability. In the previous studies, most people focused on the functional element of mRNA’s structure. But less people pay attention to the stability of the overall structure of mRNA. Therefore, this study studied the distribution law of mRNA structure’s stability in different species on the level of genomes. We also investigate the relation between free energy distribution and evolution of species affinity.In the study, the calculation of the mRNA free energy is completed using RNAfold software from Vienna RNA software package. In the research, we selected 1248 bacterial species, 34 fungi species, and 44 species of bird. And we use their protein coding sequence for calculation. When studied the birds, we managed to get 15 groups of homologous gene in pfam. The phylogenetic tree was constructed by Taxonomy, and use EvolView to edit. The distribution of free energy in different species were studied. And we found that the difference of free energy between different species can be changeable. As the evolutionary distance grows, the free energy shows certain regularity.